中国实验动物学报2017，Vol.25Issue(3)：235-240,6.DOI:10.3969/j.issn.1005-4847.2017.03.002
三种方法复制"二次打击"急性呼吸窘迫综合征动物模型的比较与评价
Comparison and evaluation of three "two-hit" animal models of acute respiratory distress syndrome induced by different methods
摘要
Abstract
Objective To find an ideal animal model of acute respiratory distress syndrome (ARDS) through investigating the characteristics of three "two-hit" animal models of ARDS.Methods Forty-eight SD rats were randomly divided into 4 groups: Control group [2.5 mL/kg normal saline (NS) i.v.given at 0 min and 30 min];OA+OA group [0.5 mL/kg oleic acid (OA) i.v.given at 0 min and 30 min];LPS+LPS group [2.5 mg/kg lipopolysaccharide (LPS) i.v.given at 0 min and 30 min];and OA+LPS group [0.5 mL/kg OA i.v.given at 0 min and 2.5 mg/kg LPS,i.v.given at 30 min].The samples were collected at 5 h after the second drug injection.White blood cells count (WBC),polymorphonuclear leukocyte ratio (PMN%),total protein concentration,tumor necrosis factor α (TNF-α) level in bronchoalveolar lavage fluid (BALF),arterial blood gas analysis and lung wet-dry weight ratio (W/D) were measured,respectively.Pathological changes in the lung tissues were observed and histological scores were evaluated.Results Compared with those in the control group,PaCO2,WBC,PMN%,total protein concentration and TNF-α levels in BALF were significantly increased,while PaO2 was dramatically decreased (P<0.01) in the OA+OA,LPS+LPS and OA+LPS groups.The levels of protein concentration in BALF and lung W/D ratio in the OA+LPS group were significantly higher than these in the LPS+LPS group (P<0.05 for all),but had no statistically significant difference compared with these in the OA+OA group.The levels of WBC,PMN% and TNF-α in BALF in the OA+LPS group were significantly higher than those in the OA+OA group (P<0.05),but not significantly different from those in the LPS+LPS group.The most typical pathological changes and the highest pathological scores were found in the OA+LPS group.Conclusions All the three different methods including OA+OA,LPS+LPS,and OA+LPS can be used to establish “two-hit” animal models of acute respiratory distress syndrome.The “two-hit” animal model of acute respiratory distress syndrome induced by OA+LPS is more closer to clinical ARDS and is useful for studies on the pathophysiology of ARDS,and is an ideal “two-hit” animal model of ARDS.关键词
急性呼吸窘迫综合征/"二次打击"模型/油酸/内毒素Key words
Acute respiratory distress syndrome/"Two-hit" animal model/Oleic acid/Lipopolysaccharide分类
生物科学引用本文复制引用
姚敦琛,潘龙,陈秉钧,余雨中,李晟杰,胡桂和,马明明,涂永生..三种方法复制"二次打击"急性呼吸窘迫综合征动物模型的比较与评价[J].中国实验动物学报,2017,25(3):235-240,6.基金项目
国家自然科学基金(编号:81302771) （编号:81302771）
广东省医学科学研究基金(编号:A2016382) （编号:A2016382）
广东省自然科学基金(编号:2014A030313087) （编号:2014A030313087）
国家级大学生创新创业训练计划项目(编号:2015105700010) （编号:2015105700010）
广州医科大学大学生科技创新项目(编号:2014A015). （编号:2014A015）
