中国实验动物学报2017，Vol.25Issue(3)：250-255,6.DOI:10.3969/j.issn.1005-4847.2017.03.004
C1型尼曼-匹克病小鼠生长繁殖及血液生理生化指标的测定分析
Analysis of growth, reproductive and blood physiological and biochemical parameters in Niemann-Pick disease type C1 mice
摘要
Abstract
Objective To investigate the growth curve,breeding rate,and blood physiological and biochemical parameters in Npc1 gene mutant mice (Npc1-/-) for providing theoretical evidence in research on Niemann-Pick disease type C1 (NPC1) patient.Methods 1) The body mass of Npc1-/-,Npc1+/-,and Npc1+/+ mice (n=120;60♀,60♂) was measured from 0 to 77 days;(2) As Npc1-/-mice were born only by the mating Npc1+/-mice,the breeding rate of Npc1+/-mice was counted here from the 1st to 4th generation;(3) The blood physiological and biochemical parameters were measured on both Npc1-/-and Npc1+/+ mice at 60 days.Results 1) Compared with the wild type controls,the body weight of Npc1-/-mice was progressively increased up to 7 weeks and then decreased,and died around 11 weeks.The body weight of the Npc1+/-and Npc1+/+ mice was increased as time went on.After 4 weeks,the male mice showed a higher weight gain than the females;(2) The generations of Npc1+/-mice had no significant difference in mating-parturition interval,litter size,weaning litter and the number of male and female (P>0.05),but the weaning rate of the 2nd generation was significantly higher than that of the 1st generation (P<0.05);(3) The hematological parameters showed a significant difference only in mean corpuscular hemoglobin (MCH) and mean peroxidase index (MPXI) between the Npc1-/-and Npc1+/+ mice (P<0.05).No significant difference was found in other hematological parameters (P>0.05).Among the biochemical parameters,aspartate aminotransferase (AST),glucose (GLU),lactate dehydrogenase (LDH),potassium (K) and copper (Cu) had a significant difference between the Npc1-/-and Npc1+/+ mice (P<0.05).Conclusions 1) The growth curves of Npc1-/-,Npc1+/-,and Npc1+/+ mice are different due to different genotype and sex;(2) The reproduction rates of Npc1+/-mice have no significant difference among different generations;(3) The blood physiological parameters (MCH,MPXI) and biochemical parameters (UREA,AST,GLU,LDH,K,Cu) are significantly different between Npc1-/-and Npc1+/+ mice.关键词
C1型尼曼-匹克病,小鼠/生长曲线/繁殖/血液参数Key words
Niemann-Pick disease type C1,Mice/Growth rate/Reproduction/Hematological parameters分类
生物科学引用本文复制引用
乔梁,杨恩慧,杨记超,顾巧美,董宏天,林俊堂,闫欣..C1型尼曼-匹克病小鼠生长繁殖及血液生理生化指标的测定分析[J].中国实验动物学报,2017,25(3):250-255,6.基金项目
国家自然科学基金项目(编号:81400936,81600987,U1304808) （编号:81400936,81600987,U1304808）
河南省高等学校重点科研项目(编号:16A180014) （编号:16A180014）
河南省高校科技创新人才支持计划(14HASTIT032) （14HASTIT032）
河南省科技发展计划项目(142300410192) （142300410192）
新乡医学院精神神经医学学科群支持计划. （）
