中国实验动物学报2017，Vol.25Issue(3)：295-300,6.DOI:10.3969/j.issn.1005-4847.2017.03.011
卵白蛋白介导白毛黑眼兔变应性鼻炎模型树突状细胞功能的改变
Functional changes of dendritic cells in the WHBE rabbits with allergic rhinitis induced by ovalbumin
摘要
Abstract
Objective To observe and compare the function of peripheral blood derived dendritic cells (DC) in white hair black eyes (WHBE) rabbits and Japanese white (JW) rabbits with allergic rhinitis (AR) induced by ovalbumin (OVA),and to explore the mechanism of sensitivity to allergen in WHBE rabbits.Methods For the AR induction,rabbits were sensitized intraperitoneally everyday with OVA emulsified in Al(OH)3 followed from day 17 onward by 5 times nasal challenges with OVA in each nostril.General symptoms and histopathological changes of the nasal mucosa were observed.Expressions of CD86 on cell surface and antigen uptake of peripheral blood-derived dendritic cells were detected by flow cytometry at 6 days of culture.The mannose receptor (MR) mRNA expression was tested by real-time PCR.Proliferation of CFSE [5-(and 6-)-carboxyfluorescein diacetate succinimidyl ester]-labelled T cells stimulated by DC were observed by flow cytometry.Results The rabbits sensitized by OVA showed typical AR symptoms and pathological changes.Expressions of CD86 on the cell surface of dendritic cells in WHBE rabbits with AR were significantly upregulated not only compared with the normal control (NC) rabbits,but also with the JW rabbits with AR (P<0.01).The result of real-time PCR assay showed that MR mRNA expression of DC in the NC group of WHBE rabbits were significantly higher than that of the JWrabbits(P<0.01).Moreover,MR mRNA expression of DCs in the WHBE rabbits with AR were not only significantly higher than that in the NC rabbits (P<0.05),but also higher than that in the JW rabbits with AR (P<0.05).Meanwhile,OVA647 internalization percentages of DCs in the WHBE rabbits with AR were not only significantly higher than that in the NC rabbits,but also obviously higher than that in the JW rabbits with AR (P<0.01).Conclusions The sensitivity of WHBE rabbits to allergen may largely depend on the function of dendritic cells with high expression of mannose receptor and their strong ability of maturation and antigen uptake.关键词
卵白蛋白/变应性鼻炎/WHBE兔/树突状细胞Key words
Ovalbumin/Allergic rhinitis/WHBE rabbits/Dendritic cells分类
生物科学引用本文复制引用
屠珏,徐孝平,谷焕鹏,陈方明,刘军平,徐剑钦..卵白蛋白介导白毛黑眼兔变应性鼻炎模型树突状细胞功能的改变[J].中国实验动物学报,2017,25(3):295-300,6.基金项目
浙江省科技计划项目(批准号:2014C37007) （批准号:2014C37007）
浙江中医药大学比较医学创新团队(XTD201301). （XTD201301）
