中国实验动物学报2017，Vol.25Issue(3)：320-324,5.DOI:10.3969/j.issn.1005-4847.2017.03.016
维生素D受体在C57BL/6小鼠十二指肠中的定位表达分析
Location and expression of vitamin D receptor in the duodenum of C57BL/6 mice at different developmental stages
摘要
Abstract
Objective To investigate the relationship of vitamin D with the intestinal development and study the expression of vitamin D receptor (VDR) in the duodenum of C57BL/6 mice at different developmental stages.Methods Quantitative PCR (qPCR),histology using H&E staining and immunofluorescence staining,and Western blotting (WB) were performed to elucidate the expression of VDR in mice intestine at different growth and developmental stages.Results The peak of VDR mRNA expression reached on 21 d.The pathological result showed that VDR mainly distributed in the cytoplasm of epithelial cells and smooth muscle cells in the mouse duodenum.WB result indicated that there was no nuclear translocation of VDR protein in the mouse duodenum.Conclusions This study demonstrates the regularity of expression of VDR in the mouse duodenum during its development,and contributes to understanding the function of VDR in the intestines.关键词
维生素D受体/十二指肠/表达/小鼠Key words
Vitamin D receptor/Duodenum/Expression/Mouse分类
生物科学引用本文复制引用
程佳,刘少伟,曹婉莹,杨祎琦,姚望..维生素D受体在C57BL/6小鼠十二指肠中的定位表达分析[J].中国实验动物学报,2017,25(3):320-324,5.基金项目
陕西省教育厅项目(No.14JK1159) （No.14JK1159）
汉中市科技局项目(No.2014ZKC47-07) （No.2014ZKC47-07）
陕理工博士启动项目(No.SLGQD13(2)-22) （No.SLGQD13(2）
大学生创新创业训练计划项目(No.UIRP15068). （No.UIRP15068）
