中国比较医学杂志2017，Vol.27Issue(6)：12-16,5.DOI:10.3969.j.issn.1671-7856.2017.06.003
五指山小型猪、西藏小型猪和巴马小型猪对外源性脂肪的敏感性观察
Observation on the sensitivity of Wuzhishan, Tibetan and Bama minipigs to exogenous fats
摘要
Abstract
Objective To observe the sensitivity of Wuzhishan, Tibetan and Bama minipigs to exogenous fats.Methods A total of 15 male minipigs including 5 WZS minipigs, 5 Tibetan minipigs and 5 Bama minipigs, were used in this study.The minipigs were intravenously injected with fat emulsion and fed with high-fat diet, and the changes of serum total cholesterol (TC), low density lipoprotein (LDL-C), high density lipoprotein (HDL-C) and triglyceride (TG) levels were detected at 0.5 h, 1 h, 1.5 h, 2 h, 2.5 h and 3 h after intravenous injection of fat emulsion and at 3 h and 12 h, 24 h, 36 h, 48 h, 60 h, 72 h and 84 h after fed with high-fat diet, respectively, and calculated the changes of area under the curve (ΔAUC) values.Results The triglyceride (TG) in the three kinds minipigs were significantly increased (P<0.01), and reached a peak at 0.5 h after injection.The degree of elevation of ΔAUC values were different showing on increasing order: Tibetan minipig > Wuzhishan minipig > Bama minipig, while TC, LDL-C and HDL-C showed no significant changes (P>0.05).Moreover, TC, LDL-C, HDL-C and TG were significantly increased in the three stocks of minipigs induced by feeding with high-fat diet (P<0.05, P<0.01).Among them, the TC and LDL-C of Wuzhishan minipigs peaked at 36 h, HDL-C peaked at 48 h and TG peaked at 24 h after feeding, respectively.TC, LDL-C and HDL-C in the Bama minipigs and Tibetan minipigs peaked at 48-60 h and TG peaked at 36 h after feeding, and the ΔAUC values of TC, LDL-C and HDL-C were in an increasing order of Wuzhishan minipigs > Bama minipigs > Tibetan minipigs.Conclusions The three stocks of minipigs are sensitive to TG after intravenous injection of fat emulsion, and the lipid tolerance values are in an order of Tibetan minipig > Wuzhishan minipigs > Bama minipigs.Meanwhile, the three stocks of minipigs are also sensitive to TC, LDL-C and HDL-C after feeding with high-fat diet, and the lipid tolerance values are in an increasing order of Wuzhishan minipig > Bama minipigs > Tibetan minipigs.关键词
小型猪/血脂代谢/脂耐量/敏感性Key words
Minipig/Blood lipid metabolism/Lipid tolerance/Sensitivity分类
医药卫生引用本文复制引用
陈亮,潘永明,朱科燕,徐孝平,徐剑钦,陈民利..五指山小型猪、西藏小型猪和巴马小型猪对外源性脂肪的敏感性观察[J].中国比较医学杂志,2017,27(6):12-16,5.基金项目
浙江省科技厅实验动物科技计划项目(2012C37078),浙江中医药大学比较医学创新团队(XTD201301). （2012C37078）
