中国比较医学杂志2017，Vol.27Issue(6)：33-36,45,5.DOI:10.3969.j.issn.1671-7856.2017.06.007
鼠伤寒沙门氏菌S.Typhimurium感染C57BL/6小鼠肠道模型的建立及表型分析
Etablishment and phenotypic analysis of a C57BL/6 mouse model of S.Typhimurium infection
摘要
Abstract
Objective To establish a C57BL/6 mouse model of intestinal infection induced by S.Typhimurium.Methods In order to improve the infectious sensitivity of S.Typhimurium, C57BL/6 mice were intragastrically given 5% (w/v) NaHCO3.Then mice were challenged with S.Typhimurium.The health condition, survival and body weight of mice were observed from day 0 to day 7 after the bacterial infection.The pathological changes were also examined.Results the mice challenged with S.Typhimurium showed decreased body weight and typical clinical signs, including in appetence, piloerection and low survival rate.Macroscopic dissection revealed that intestinal hyperemia and swelling were founded in the mice challenged with S.Typhimurium.Histopathology showed intestinal epithelial and mucosal damages.Conclusions We have successfully established a C57BL/6 mouse model of S.Typhimurium infection.This model may be of crucial significance for studying the biological functions of associated immunological molecules or cytokines in the process of inflammatory bowel disease induced by S.Typhimurium.关键词
肠道感染/S.Typhimurium/C57BL/6/组织病理Key words
Intestinal infection/S.Typhimurium/C57BL/6 mouse/Histopathology引用本文复制引用
韦荣飞,李梦媛,徐大模,高苒..鼠伤寒沙门氏菌S.Typhimurium感染C57BL/6小鼠肠道模型的建立及表型分析[J].中国比较医学杂志,2017,27(6):33-36,45,5.基金项目
国家自然科学基金(81601375),协和青年基金资助,中央高校基本科研业务费专项资金资助(3332016077),中国医学科学院医学实验动物研究所基本科研业务费专项资助(2016ZX310033). （81601375）
