中国比较医学杂志2017，Vol.27Issue(6)：37-45,9.DOI:10.3969.j.issn.1671-7856.2017.06.008
颈内动脉刺破法制作大鼠蛛网膜下腔出血模型
Preparation of a rat model of subarachnoid hemorrhage by a fiber core inserted in the internal carotid artery
摘要
Abstract
Objective To report a newly developed method and procedure to establish a rat model of subarachnoid hemorrhage in detail, and to provide a better model simulating the clinical subarachnoid hemorrhage caused by a ruptured aneurysm for related research.Methods One hundred and twenty healthy SPF 2-3-month old male Sprague-Dawley rats were divided into 4 groups, 30 rats in each group.The three experimental groups were sacrificed at 6, 24 and 72 hours after modeling.Rat models of subarachnoid hemorrhage were established by inserting a fiber core in the internal carotid artery and piercing this artery.Successful establishment of the subarachnoid hemorrhage model was confirmed by observation of breathing, pupil, defecation, urination and inspection at autopsy dissection.The controllability and reproducibility of this model were verified by observation of clinical manifestation and explored by mortality analysis.Results Subarachnoid hemorrhage was successfully induced by fiber core piercing the internal carotid artery at the needed location.Conclusions This method of model preparation is stable and understandable.The operation is nimble, with a good reproducibility.This model can be successfully performed after a short time learning, well simulate the sudden hemorrhage caused by a ruptured aneurysm, and suitable for research on early brain injury and vasospasm after subarachnoid hemorrhage.关键词
蛛网膜下腔出血/血管穿刺法Key words
Subarachnoid hemorrhage (SAH)/Aneurysm/Internal carotid artery/Surgery/Animal model/Rat/Fiber core引用本文复制引用
刘振刚,高建亮,孙林林,郭鑫,王萌,庞智寅,付爱军,李建民,田景瑞..颈内动脉刺破法制作大鼠蛛网膜下腔出血模型[J].中国比较医学杂志,2017,27(6):37-45,9.基金项目
河北省省级重大医学项目(Zd2013093). （Zd2013093）
