中国动物传染病学报2017，Vol.25Issue(3)：23-27,5.
伪狂犬病病毒变异株gE蛋白单克隆抗体的制备与鉴定
GENERATION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST GE PROTEIN OF PSEUDORABIES VIRUS VARIANT STRAIN
摘要
Abstract
The gene encoding the key antigenic epitope regions of gE of pseudorabies virus (PRV) was amplifi ed in PCR and inserted into the pCold-TF expression vector. Expression of the recombinant fusion protein about 90 kDa was observed under 1mmol/L IPTG environment. The recombinant gE protein purified with nickel ion metal affinity chromatography was used to immunize 6-week-old BALB/c female mice for the preparation of monoclonal antibodies (McAbs). Two hybridoma cell lines (5B2 and 6E6) were obtained for stably producing anti-gE protein McAb. These two McAbs reacted with PRV variant strain (JS-2012) but not with the gE deleted vaccine virus Bartha-K61 strain in indirect immunofl uorescence assay(IFA). Western blot results showed that 5B2 and 6E6 did not react with PRV(JS-2012). The results indicated that 5B2 and 6E6 might be against confomational epitope of gE. These specifi c monoclonal antibodies might provide a good tool for the development of PRV diagnostic method.关键词
伪狂犬病毒/变异株/gE蛋白/单克隆抗体Key words
Pseudorabies virus/variant strain/gE protein/monoclonal antibodies分类
农业科技引用本文复制引用
武吉强,徐晶晶,童武,王涛,叶超,郑浩,单同领,童光志,李国新..伪狂犬病病毒变异株gE蛋白单克隆抗体的制备与鉴定[J].中国动物传染病学报,2017,25(3):23-27,5.基金项目
上海市闵行区人才发展专项基金 （）
上海市自然科学基金项目(14ZR1448900) （14ZR1448900）
上海市科技兴农重点攻关项目(沪农科攻字(2015)第1-7号, 沪农科攻字(2016)第4-2号) （沪农科攻字(2015）
国家生猪现代产业技术体系项目(CARS-36) （CARS-36）
