中国动物传染病学报2017，Vol.25Issue(3)：28-33,6.
三源重组H1N1亚型猪流感病毒反向遗传操作平台的建立
ESTABLISHMENT OF REVERSE GENETICS SYSTEM OF TRIPLE-REASSORTANT H1N1 SUBTYPE SWINE INFLUENZA VIRUS
摘要
Abstract
The eight gene segments of triple-reassortant H1N1 subtype Swine influenza virus A/swine/Tianjin/10/2013(H1N1) were amplifi ed in RT-PCR and individually cloned into the transcription/ expression vector pBD that was used to transfect 293T cells. The supernatant samples of transfected 293T cells were collected after 48 h and inoculated into MDCK cells. The rescued viruses was determined to Swine infl uenza virus in hemagglutination test. The full genome of the rescued virus was confi rmed no nucleic acid change as compared with the wild-type virus through sequence analysis. The measured results of growth curves showed no signifi cant differences in virus titer between two strains. Therefore, it concluded that the virus was rescued successfully. The establishment of reverse genetic system of H1N1 subtype Swine infl uenza virus settles the foundation for future research on biological characteristics and production of recombinant infl uenza vaccine of H1N1 swine infl uenza.关键词
猪流感病毒/H1N1/反向遗传操作技术/病毒拯救Key words
Swine infl uenza virus/H1N1/reverse genetics/virus rescue分类
农业科技引用本文复制引用
汪琪,刘晓敏,杨海明,王帅勇,单同领,童武,李国新,童光志,于海..三源重组H1N1亚型猪流感病毒反向遗传操作平台的建立[J].中国动物传染病学报,2017,25(3):28-33,6.基金项目
上海市自然科学基金青年项目(16ZR1444000) （16ZR1444000）
中央级公益性科研院所基本科研业务费项目(2015JB07) （2015JB07）
闵行领军人才队伍建设专项资金 （）
中国农业科学院创新工程"猪病毒性繁殖障碍综合症团队"项目 （）
