中国动物传染病学报2017，Vol.25Issue(3)：68-72,5.
日本血吸虫凋亡相关基因SjBAK的初步研究
A PRELIMINARY STUDY ON APOPTOSIS ASSOCIATE GENES SJBAK OF SCHISTOSOMA JAPONICUM
摘要
Abstract
The objectives of the present study were to clone the apoptosis related gene ofSchistosoma japonicumSjBAK(Bcl-2 homologous antagonist/killer), analyze its expression in schistosome worms and possible role in the development of schistosome. The full-length sequence ofSjBAK was cloned in PCR and the recombinant plasmid containingSjBAK was constructed. The expression of SjBAK inS. japonicum at different development stages from non-susceptible host rats and susceptible host mice were analyzed using Real-time PCR. The ORF of theSjBAK gene contained 2142 bp encoding 713 amino acids with the theoretical molecular weight of 79.3 kDa and theoretical isoelectric point at 4.9. Analysis also revealed that the molecule possessed at least three BH domains and belonged to the member of Bcl family. Real-time PCR analysis showed that the gene expressed in all worms at four development stages and the expression level in worms from rats was higher than those from mice. Signifi cant difference inSjBAK expression was observed in worms from two different hosts at 32 d and 42 d post-infection(P<0.05). In conclusion, the apoptosis-related geneSjBAK ofS japonicum may play an important role in the development of Schistosome.关键词
日本血吸虫/BAK/细胞凋亡Key words
Schistosoma japonicum/BAK/apoptosis分类
农业科技引用本文复制引用
窦雪峰,林矫矫,王涛,吕超,韩倩,曹晓丹,沈元曦,张祖航,洪炀,傅志强..日本血吸虫凋亡相关基因SjBAK的初步研究[J].中国动物传染病学报,2017,25(3):68-72,5.基金项目
国家自然科学基金(81271871) （81271871）
