中国兽医科学2017，Vol.47Issue(6)：694-700,7.DOI:10.16656/j.issn.1673-4696.2017.06.004
兔出血症病毒TaqMan荧光定量PCR检测方法的建立
Establishment of TaqMan real-time PCR for the detection of rabbit hemorrhagic disease virus
摘要
Abstract
To establish TaqMan real-time PCR for detecting rabbit hemorrhagic disease virus(RHDV) rapidly,a pair of specific primers and a probe were designed based on the conservative gene VP60 of RHDV.The RHDV TaqMan real-time PCR assay was established by the conditions optimization,repeatability,sensitivity and specificity test.In results,standards had good liner relations in the concentration of 2.8 × 106to 2.8 × 102copies/μL,the limited detection content was 2.8 × l01copies/μL and the variation coefficient was less than 2％.In conclusion,the specificity,sensitivity and repeatability of the method satisfy the experimental design requests,and the method can rapidly detect the clinical RHDV samples,providing a reliable technical tool for detection and the quantitative of RHDV in our cou ntry.关键词
兔出血症病毒/TaqMan/荧光定量PCRKey words
rabbit hemorrhagic disease/TaqMan/real-time quantitative PCR分类
农业科技引用本文复制引用
蒙正群,李桂黎,周丽军,冷依伊,罗怡琳,刘亚东,王印,姚学萍,杨泽晓..兔出血症病毒TaqMan荧光定量PCR检测方法的建立[J].中国兽医科学,2017,47(6):694-700,7.基金项目
国家自然科学基金项目(31402222) （31402222）
“十二五”国家科技支撑计划项目(2013BAD12B04) （2013BAD12B04）
四川省科技支撑计划项目(2016N20002) （2016N20002）
