中国兽医科学2017，Vol.47Issue(6)：707-712,6.DOI:10.16656/j.issn.1673-4696.2017.06.006
猪细小病毒病毒样颗粒的体外组装
Self-assembly of virus-like particles of porcine parvovirus
摘要
Abstract
In order to develop VLPs vaccines against porcine parvovirus,the PPV VP2 gene was cloned into a pSMKexpression vector containing a His tag and small ubiquitin-like modifier(SUMO) tag that can promote the soluble expression of PPV VP2 proteins in Escherichia coli cells.After removal of the SUMO tag from the expressed recombinant fusion protein,the recombinant PPV VP2 protein had self-assembled into VLPs.Further study showed that the self-assembly efficiency of VLPs was affected by different pH levels and ionic strengths.In conclusion,PPV VLPs with highly reactionogenicity were obtained by the prokaryote expression system,which may pave a new way for research and development of a VLPs vaccine against PPV.关键词
猪细小病毒/病毒样颗粒/原核表达/VP2基因Key words
porcine parvovirus/virus-like particles/prokaryotic expression/VP2 gene分类
农业科技引用本文复制引用
宋品,孙世琪,董虎,滕志东,曹随忠,郭慧琛,余树民..猪细小病毒病毒样颗粒的体外组装[J].中国兽医科学,2017,47(6):707-712,6.基金项目
国家“十二五”科技支撑计划项目(2013BAD12B00) （2013BAD12B00）
国家国际科技合作专项(2014DFA31890) （2014DFA31890）
国家自然科学基金项目(31672592) （31672592）
