中国兽医科学2017，Vol.47Issue(6)：713-720,8.DOI:10.16656/j.issn.1673-4696.2017.06.007
猪伪狂犬病病毒流行毒株gC基因的分子特征及其对小鼠的毒力试验
Molecular characterization of gC gene of pseudorabies virus epidemic isolates and their virulence test in mice
摘要
Abstract
To elucidate the molecular characterization of the gC gene of pseudorabies virus(PRV) epidemic isolates and their virulence in mice,six PRV isolates from clinical cases at different areas were isolated to be used for the genetic variation analysis and virulence test in BALB/c mice.The gC amino acid sequences of the six PRV isolates and other thirty sequences of PRY strains available in GenBank were used for analysis and comparison.The results suggested that the six isolates and PRV variants isolated in China since 2011 belonged to the same branch,and shared from 98.7％ to 100％ identity with PRV variants,but only from 93.2％ to 94.0％ identity with the classic strain PRV-SC.A hypervariable region located in No.52-70 aa was found on the gC protein for the six PRV isolates,especially the insert sequences of seven amino acids(64AAASTPA70).The analysis of the gC protein glycosylation sites showed that six 0-linked glycosylation sites were added compared with the classic strain PRV-SC.Furthermore,one mutation of P69R and two mutations of P80Q and N83G were found in the B cell epitopes of gC,respectively.Meanwhile,two mutations were found in the heparin binding domain(HBD),of which the fifth amino acid was mutated into Q,and the third amino acid was mutated into C.BALB/c mice were inoculated with the six PRV isolates to evaluate their virulence.The results indicated that the LD50 of PRV-HRB and PRV-SH isolates was 1 × 103.21/mL,PRV-DL,PRV-JL and PRV-GZL were 1 × i03.75/mL in LD50,while PRV-JFwas 1 × 104.32/mLin LD50.This study result illuminated the correlation between the molecular characterization of the gC gene of PRV new epidemic isolates and their virulence in mice,which provided a scientific basis for studying the pathogenesis of the virus.关键词
伪狂犬病病毒/流行毒株/gC基因分子特征/毒力Key words
pseudorabies virus/epidemic isolates/molecular characterization of the gC gene/virulence分类
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夏德利,黄立平,危艳武,王一平,杜文娟,谢永兴,吴洪丽,冯力,刘长明..猪伪狂犬病病毒流行毒株gC基因的分子特征及其对小鼠的毒力试验[J].中国兽医科学,2017,47(6):713-720,8.基金项目
黑龙江省自然科学基金项目(C2015064) （C2015064）
哈尔滨市应用技术研究和开发项目(2015RAQYJ066) （2015RAQYJ066）
