中国兽医科学2017，Vol.47Issue(6)：727-732,6.DOI:10.16656/j.issn.1673-4696.2017.06.009
猪圆环病毒2型Cap基因的原核表达及IgY多抗的制备
Prokaryotic expression of capsid gene of porcine circovirus 2 and preparation of polyclonal IgY antibodies against PCV2 Cap protein
摘要
Abstract
In the experiment,the PCV2 Cap gene was inserted into the pET 32a vector to construct recombinant plasmid pET-32a-Cap and was expressed in host bacterium BL21.The recombinant Cap protein was identified by SDS-PAGE and was purified by Ni+ affinity column and then immunized with laying hens to prepare anti-Cap polyclonal antibodies IgY.Antibody titers and binding of IgY against Cap of PCV2 was determined by indirect ELISA.The results revealed that Cap protein was successfully expressed in prokaryotic cells.The recombinant protein was mainly solubly expressed in the supernatant of cell lysate.The titer of the egg yolk antibody was up to 1 ∶64 000 and the prepared IgY antibody has higher binding capacity with PCV2 and Cap protein.This study result laid the foundation for the development of a PCV2 Cap protein kit.关键词
猪圆环病毒2型/重组Cap蛋白/原核表达/IgY抗体Key words
porcine circovirus type 2(PCV2)/recombinant Cap protein/prokaryotic expression/IgY分类
农业科技引用本文复制引用
张遥遥,张小莺,李新平..猪圆环病毒2型Cap基因的原核表达及IgY多抗的制备[J].中国兽医科学,2017,47(6):727-732,6.基金项目
陕西省国际科技合作基地建设项目(2015SD0018) （2015SD0018）
