中国兽医科学2017，Vol.47Issue(6)：733-738,6.DOI:10.16656/j.issn.1673-4696.2017.06.010
乳酸菌复合制剂对柔嫩艾美耳球虫感染雏鸡免疫水平的影响及免疫保护效果评价
Evaluation of immune protective effects of Lactobacillus compound preparation against Eimeria tenella infection in chickens
摘要
Abstract
In order to investigate the role and immune effects of Lactobacillus against Eimeria tenella in broiler chickens,150 one day-old broiler chickens were randomly divided into three groups in this study.One group was feed with 2×108CFU/200 μL compound preparation(Lactobacillusrhamnosus,Pediococcus acidilactici,Lactobacillus plantarum),the negative and positive control groups were set at the same time.The levels of cytokines IFN-γ,IL-2,IgG and SIgA,the proliferation of lymphocytes and the number of CD4+ and CD8+ T]ymphocytes in peripheral blood were detected before and after challenge with Ktenella oocysts.Additionally,the body weight gain,the number of oocysts shedding and cecal lesion also were detected.The results indicated that the preparation enhanced the cellular and humoral immunity by significantly increasing IFN-γ,IL-2,IgG,SIgA levels and the number of T lymphocytes in peripheral blood(P＜0.05).Furthermore,theeffectofweight loss onchickens infected withEimeria oocysts,the number of oocysts shedding and cecal pathological injury were reduced significantly by the compound preparation(P＜0.05).Therefore,it concludes that probiotics is expected to become a new preventive method of chicken coccidiosis.关键词
柔嫩艾美耳球虫/乳酸菌/细胞免疫/体液免疫/保护效果Key words
Eimeria tenella/Lactobacillus/cellular immunity/humoral immunity/protective effect分类
农业科技引用本文复制引用
杨桂连,杨军,黄海斌,杨文涛,姜延龙,石春卫,王春凤..乳酸菌复合制剂对柔嫩艾美耳球虫感染雏鸡免疫水平的影响及免疫保护效果评价[J].中国兽医科学,2017,47(6):733-738,6.基金项目
国家高技术研究发展计划(863)项目(2013AA102806) （863）
国家自然科学基金项目(31272552,31272541,31672528) （31272552,31272541,31672528）
吉林省科技发展计划项目(20160519011JH) （20160519011JH）
吉林省产业创新专项资金项目(2016C063) （2016C063）
