中国兽医科学2017，Vol.47Issue(6)：762-767,6.DOI:10.16656/j.issn.1673-4696.2017.06.015
新城疫病毒溶瘤株SH15全基因组序列的测定及分析
Whole genome sequencing and biological characterization of a new oncolytic Newcastle disease virus SH15
摘要
Abstract
The oncolytic strain of Newcastle disease virus(NDV)SHl5 was used as material due to its good oncolytic effect on LoVo cell and A549 cell in a time-dependent manner.Ten pairs of primers were designed according to Mukteswar strain in GenBank and positive clones were screened by RT-PCR.Sequence analysis showed that the length of SH15 was 15 186 nucleotides(GenBank accession No.KY247177),and pat hogenicity tests indicated SH15 belonged to Mesogenic strain.Phylogenetic analysis showed SH15 was classified as genotype Ⅲ of Class Ⅱ,and its homology with JS/9/0S/Go and Mukteswar strain was up to 99.8％.The H203 site of HNprotein may be the key to oncolytic effect of SH15.The present study facilita tes the further studies on the new isolated NDV strain,even development of antineoplastic targeted drugs based on NDV.关键词
新城疫病毒/溶瘤/全基因测序/基因Ⅲ型/HN蛋白Key words
Newcastle disease virus(NDV)/oncolytic/genome/sequence analysis/genotype Ⅲ分类
农业科技引用本文复制引用
段坦然,唐兆新,孟春春,车路平,廖瑛,仇旭升,谭磊,孙英杰,宋翠萍,丁铲..新城疫病毒溶瘤株SH15全基因组序列的测定及分析[J].中国兽医科学,2017,47(6):762-767,6.基金项目
国家自然科学基金项目(31572585) （31572585）
