中国兽医科学2017，Vol.47Issue(6)：798-804,7.DOI:10.16656/j.issn.1673-4696.2017.06.021
ERK信号在玉米赤霉烯酮诱导的睾丸支持细胞自噬中作用的研究
Study on functions of ERK signal pathways in ZEA-induced autophagy of sertoli cells
摘要
Abstract
To elucidate the mechanism of zearalenone(ZEA) on male reproductive toxicity,we investigated the effects of ZEA on the autophagy and related signaling pathways in sertoli cells.TM4 cells were used as materials treated with 0(control group),0.1,1,10,20,30 μmol/L ZEA respectively for 24 h.With the help of the CCK-8,1aser confocal microscopy,transmission electron microscopy and West ern blot technique,the cell viability,autophagy,effects of the MAPK signaling pathway and the relationship between MAPK signal pathway and the autophagy of TM4 were investigated.The immunofluorescence results showed that with the increasing concentration of ZEA,the number of the LC3 accumulation in TM4 cells significantly increased and the number of acidicvesicles also increased.The Western-blot results showed that the expression of autophagy related protein such as LC3 Ⅱ,Beclin-1,ATG5,ATG7 was significantly increased,while the expression of P62 was significantly decreased(P＜0.05 or P＜0.01).The phosphorylation level of the ERK1/2 in the pathway of KAPK signaling increased significantly(P＜ 0.05 or P＜0.01).After adding the U0126 (ERK phosphorylation inhibitor),the level of ZEA induced au tophagy in TM4 decreased significantly (P＜0.05 or P＜0.01).The results showed that zearalenone could induce autophagy of TM4 cells in a certain concentration range,which was associated with the activation of ERK signaling pathway.关键词
玉米赤霉烯酮/睾丸支持细胞/TM4细胞系/自噬/ERKKey words
zearalenone(ZEA)/sertoli cells/TM4 cells/autophagy/ERK分类
农业科技引用本文复制引用
王冰洁,刘宗平,卞建春,冯楠楠,郑王龙,薛画眉,达剑森,邹辉,顾建红,袁燕,刘学忠..ERK信号在玉米赤霉烯酮诱导的睾丸支持细胞自噬中作用的研究[J].中国兽医科学,2017,47(6):798-804,7.基金项目
国家重点研发计划支持项目(2016YFD0501208) （2016YFD0501208）
江苏高校优势学科建设工程资助项目(PAPD) （PAPD）
2016年江苏省普通高校学术学位研究生创新计划项目(KYLX16_1406) （KYLX16_1406）
江苏省大学生实践创新训练计划项目(x20160702) （x20160702）
