中国现代医学杂志2017，Vol.27Issue(10)：1-5,5.DOI:10.3969/j.issn.1005-8982.2017.10.001
灯盏花素对肝癌细胞HepG2和结肠癌细胞Caco2的体外抗肿瘤活性筛选
Screening of in vitro anti-tumor activity of breviscapine on HepG2 and Caco2 cell lines
摘要
Abstract
Objective To investigate the inhibitory effects of breviscapine on in vitro proliferation of human hepatocellular carcinoma cell line HepG2 and colon cancer cell line Caco2.Methods Both HepG2 and Caco2 cell lines were treated with breviscapine at different concentrations for 24,48 and 72 h,and the inhibitory rate was analyzed by MTT assay.The 50％ inhibitory concentration (IC50) and the therapeutic index (TI) were used as indicators to assess their anti-tumor activity and safety.Results Breviscapine at high concentration displayed inhibitory effect on HepG2;when the concentration was 20,000 μmol,its inhibitory rate to HepG2 cells after 24,48 and 72 h was 37％,63％ and 69％ respectively.As to Caco2 cells breviscapine showed concentration dependency;when the concentration was 5,000 μ mol,its inhibitory rate after 24,48 and 72 h was 72％,95％ and 92％ respectively.The IC50 of breviscapine on HepG2 cells after 24,48 and 72 h was 24,320.5 μmol,30,667.7 μmol and 15,586.1 μmol respectively;while that on Caco2 cells after 24,48 and 72 h was 11,417.0 μmol,832.2 μmol and 1,369.2 μmol respectively.Conclusions Breviscapine exhibits different degree of inhibitory effect on HepG2 and Caco2 cells,the effect is more significant on Caco2 cells.关键词
灯盏花素/HepG2细胞/Caco2细胞/MTT法/抗肿瘤活性Key words
breviscapine/HepG2 cell/Caco2 cell/MTT assay/anti-tumor activity分类
医药卫生引用本文复制引用
曹昌娥,赖泳,杜一民..灯盏花素对肝癌细胞HepG2和结肠癌细胞Caco2的体外抗肿瘤活性筛选[J].中国现代医学杂志,2017,27(10):1-5,5.基金项目
国家自然科学基金(No:81241139) （No:81241139）
国家自然科学基金(No:81360511) （No:81360511）
