中国现代医学杂志2017，Vol.27Issue(10)：6-13,8.DOI:10.3969/j.issn.1005-8982.2017.10.002
黄芩素联合U0126诱导人乳腺癌细胞株MCF-7凋亡的分子机制研究
Mechanisms of apoptosis of human breast cancer cell line MCF-7 induced by baicalein and U0126
摘要
Abstract
Objective To investigate the molecular mechanisms of apoptosis of human breast cancer cell line MCF-7 induced by baicalein and U0126.Methods The human breast cancer cell line MCF-7 cells were treated by 20 μM baicalein,10 μM U0126,and 20 μM baicalein combined with 10 μM U0126 respectively.Flow cytometry was used to test the changes of MCF-7 cell cycle.CCK8 assay was applied to measure proliferation of MCF-7 cells.The number of cells was observed under light microscope.Flow cytometry and TUNEL were used to evaluate the apoptosis of MCF-7 cells.RT-PCR and Western blot were adopted to detect the mRNA and protein levels of apoptosis-related proteins.Results Compared to the MCF-7 cells stimulated with 20 μM baicalein alone,the ratio of S phase MCF-7 cells decreased after treatment by baicalein combined with U0126 (P ＜ 0.05).When the MCF-7 cells were treated by different concentrations of baicalein or U0126,the proliferation was inhibited dramatically in a dose-dependent way (P ＜ 0.05).Compared to the MCF-7 cells treated with baicalein alone,the early and late apoptotic cells were augmented dramatically when they were treated by both baicalein and U0126 (P ＜ 0.05).When the MCF-7 cells were treated by baicalein combined with U0126,the level of Bcl-2 decreased (P ＜ 0.05),the level of Bax greatly increased (P ＜ 0.05),and the phosphorylation levels of ERK1/2,GSK-3β and P38 obviously reduced (P ＜ 0.05) compared with the cells with single treatment of baicalein or U0126.Conclusions Baicalein and U0126 can dramatically inhibit the proliferation and increase the apoptosis of MCF-7 cells through decreasing the level of Bcl-2,reducing the phosphorylation levels of ERK1/2,GSK-3β and P38,and increasing the level of Bax.Hence,baicalein and U0126 can be used to treat breast cancer,both are very important in clinic.关键词
黄芩素/U0126/人乳腺癌细胞株MCF-7/凋亡/分子机制Key words
baicalein/U0126/human breast cancer cell line MCF-7/apoptosis/molecular mechanism分类
医药卫生引用本文复制引用
安宏元,向川南,喻小兰,唐利,李燕,唐小平,张宇骄,夏纪毅..黄芩素联合U0126诱导人乳腺癌细胞株MCF-7凋亡的分子机制研究[J].中国现代医学杂志,2017,27(10):6-13,8.基金项目
四川省科技厅项目(14JC01353-LH67) （14JC01353-LH67）
四川省泸州市科技局项目(2014-S-44) （2014-S-44）
