中国现代医学杂志2017，Vol.27Issue(10)：14-19,6.DOI:10.3969/j.issn.1005-8982.2017.10.003
MicroRNA-630对三阴性乳腺癌细胞增殖的影响及其机制研究
miR-630 inhibits proliferation of triple-negative breast cancer MDA-MB-231 cells by targeting Sox4
摘要
Abstract
Objective To investigate the effect of miR-630 on migration and invasion of triple-negative breast cancer cell line MDA-MB-231 cells and the mechanism.Methods miR-630 was transfected into MDA-MB-231 cells.MTT was employed to test the proliferation ability of MDA-MB-231 cells.Western blot was used to investigate the expressions of P27,P21 and Sox4 in MDA-MB-231 cells.Luciferase assay was used to confirm whether 3’-UTR of sex determining region Y-box 4 (Sox4-3’-UTR) was the target gene of miR-630.Results Compared with the control group,the Ki67 and Sox4 expressions and the proliferation activity of MDA-MB-231 cells were decreased,and the P27 expression was increased in the miR-630 group (P ＜ 0.05).The luciferase activity of the Sox4-3’-UTR plasmid was significantly suppressed by miR-630 (P ＜ 0.05).Over-expression of Sox4 could reverse the effect of miR-630 on MDA-MB-231 cells.Conclusions miR-630 inhibits the proliferation of triple-negative breast cancer cells by targeting Sox4-3’-UTR.关键词
三阴性乳腺癌/微小RNA630/Sox4/增殖Key words
triple-negative breast cancer/miR-630/sex determining region Y-box 4/proliferation分类
医药卫生引用本文复制引用
石岩,秦岩,宋磊,梁月勉,王小磊,韩贵良..MicroRNA-630对三阴性乳腺癌细胞增殖的影响及其机制研究[J].中国现代医学杂志,2017,27(10):14-19,6.基金项目
河北大学附属医院青年科研基金(No:2015Q014) （No:2015Q014）
