中国现代医学杂志2017，Vol.27Issue(10)：25-29,5.DOI:10.3969/j.issn.1005-8982.2017.10.005
MicroRNA-101对上皮性卵巢癌耐药细胞SKOV3/DDP顺铂敏感性的影响
Effect of miRNA-101 on chemosensitivity of SKOV3/DDP cells to Cisplatin
摘要
Abstract
Objective To explore the effect of miRNA-101 on Cisplatin sensitivity of chemo-resistant epithelial ovarian cancer SKOV3/DDP cells and the related mechanism.Methods In this study,the expression of miRNA-101 in SKOV3/DDP cells was up-regulated by transfection.Meanwhile,RT-PCR assay was applied to detect the expressions of miRNA-101 mRNA and BDNF mRNA,and CCK-8 kit was used to measure the Cisplatin sensitivity and proliferative ability of SKOV3/DDP cells.In addition,the apoptosis of SKOV3/DDP cells was detected by Annexin V-FITC/PI flow cytometry.Results Compared with the control group,the expression of miRNA-101 and the apoptosis rate of miRNA-101 in the transfected group increased significantly [(1.000 ± 0.022) vs (5.380 ± 0.246),P ＜ 0.05;(11.020 ± 0.685) ％ vs (26.158 ± 1.278) ％,P ＜ 0.05].The expression of BDNF mRNA decreased significantly [(1.000 ± 0.042) vs (0.389 ± 0.055),P ＜ 0.05],and the Cisplatin IC50 and the proliferative ability of SKOV3/DDP cells were obviously decreased after transfection [(57.276 ± 1.717) vs (33.176 ± 2.465),P ＜ 0.05;(1.776 ± 0.030) vs (1.642 ± 0.252),P ＜ 0.05].Conclusions miRNA-101 could effectively enhance the Cisplatin sensitivity and promote the apoptosis of SKOV3/DDP cells.关键词
miRNA-101/卵巢癌/顺铂/BDNF mRNA/增殖/细胞凋亡Key words
miRNA-101/epithelial ovarian cancer/Cisplatin/BDNF mRNA/proliferation/apoptosis分类
医药卫生引用本文复制引用
代娟,韩萍,曹燕花,王秀娟,杨莉,于笑涵..MicroRNA-101对上皮性卵巢癌耐药细胞SKOV3/DDP顺铂敏感性的影响[J].中国现代医学杂志,2017,27(10):25-29,5.基金项目
河北省临床医学优秀人才培养(No:310103020704) （No:310103020704）
