中国现代医学杂志2017，Vol.27Issue(10)：30-34,5.DOI:10.3969/j.issn.1005-8982.2017.10.006
抑制DBC2基因启动子甲基化促进乳腺癌细胞凋亡的研究
Expression of DBC2 gene in breast cancer cells and its effect on cell proliferation and apoptosis
樊艳1
作者信息
- 1. 河南省中医院乳腺科,河南郑州450002
- 折叠
摘要
Abstract
Objective To study the association between expression of DBC2 gene and its promoter methylation,and the effect of DBC2 on the proliferation and apoptosis of breast cancer T-47D cells.Methods Three breast cancer cell lines (T-47D,MCF-7 and MDA-MB-23) and normal breast cell line (MCF-10) were cultured in vitro,the expression and promoter methylation status of DBC2 in the four cell lines were measured.T-47D cells were treated by 5-Aza-CdR,and the effect of 5-Aza-CdR on the DBC2 mRNA level and promoter methylation status were detected.T-47D cell proliferation and apoptosis were determined by MTT and Annexin-V/PI respectively.Results DBC2 gene expression was not observed,whereas DBC2 promoter methylation was found in T-47D cells.5-Aza-CdR reversed the methylation of DBC2 promoter and activated the expression of DBC2 (P ＜ 0.05).The cell proliferation was significantly inhibited and the apoptosis was promoted in T-47D cells after treatment with 5-AzaCdR (P ＜ 0.05).Conclusions The methylation of DBC2 promoter induces the silence of DBC2 mRNA.The activation of DBC2 gene expression can inhibit the cell proliferation and promote apoptosis of T-47D cell.关键词
乳腺癌/DBC2/甲基化/5-Aza-CdRKey words
breast cancer/DBC2/methylation/5-Aza-CdR分类
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樊艳..抑制DBC2基因启动子甲基化促进乳腺癌细胞凋亡的研究[J].中国现代医学杂志,2017,27(10):30-34,5.
