中国现代医学杂志2017，Vol.27Issue(10)：40-46,7.DOI:10.3969/j.issn.1005-8982.2017.10.008
硫化氢提高肠道微循环灌注保护新生儿坏死性小肠结肠炎
Exogenous H2S up-regulates intestinal microcirculation to protect rats from neonatal necrotizing enterocolitis
张丙宏 1陶弯 1钟森 2唐俊明3
作者信息
- 1. 武汉大学人民医院儿科,湖北武汉430060
- 2. 湖北医药学院附属人民医院,湖北十堰442000
- 3. 湖北医药学院附属人民医院研究所,湖北十堰442000
- 折叠
摘要
Abstract
Objective To research the effect of H2S on intestinal microcirculation of newborn SD rats with neonatal necrotizing enterocolitis (NEC).Methods Ten newborn Sprague-Dewley (SD) rats were used to detect the expression of H2S synthase.Eighty SD rats were randomly divided into 4 groups:control group in which the rats were treated with 0.9％ NaC1,NEC group in which the rats were treated with LPS+0.9％ NaC1,NEC+GYY4137 group in which the rats were treated with LPS+ GYY4137 and NEC+AOAA group in which the rats were treated with LPS+ AOAA.GYY4137 is the donor of H2S and AOAA is the inhibitor of cystathionine β-synthase (CBS).The NEC model of SD rats was induced by bottle-feeding,hypoxia treatment,cold stimulation,and lipopolysaccharide (LPS)peritoneal injection for 3 days.Then HE stain,the Histologic Injury Score and photos taken under animal microscope were used to show the injury of intestinal tissues.What's more,the intestinal microvascular blood flow was detected by real-time imaging system of FLPI2 laser speckle blood flow and the survival time of the newborn rats was surveyed.The protective effect of H2S on intestinal microcirculation of newborn NEC rats was explored.Results Compared with the NEC group,the intestinal microvascular blood flow was significantly increased and the Histologic Injury Score was significantly decreased in the NEC +GYY4137 group (P ＜ 0.05).Importantly,administration of AOAA to the NEC rats significantly increased the Histologic Injury Score (P ＜ 0.05),but there was no significant difference in the intestinal microvascular blood flow between the NEC group and the NEC+ AOAA group.When compared to the control group,the intestinal microvascular blood flow was significantly decreased and the Histologic Injury Score was significantly increased in the NEC group.Conclusions H2S [7.5 mg/(kg ·d)] could protect newborn NEC rats and increase the survival rate by increasing blood flow of intestinal microcirculation.关键词
新生儿坏死性小肠结肠炎/硫化氢/微循环灌注Key words
neonatal necrotizing enterocolitis/hydrogen sulfide/intestinal microcirculation分类
医药卫生引用本文复制引用
张丙宏,陶弯,钟森,唐俊明..硫化氢提高肠道微循环灌注保护新生儿坏死性小肠结肠炎[J].中国现代医学杂志,2017,27(10):40-46,7.
