中国烟草科学2017，Vol.38Issue(3)：74-79,6.DOI:10.13496/j.issn.1007-5119.2017.03.013
烟草内生真菌多样性及其细胞毒活性初步研究
The Biodiversity of Endophytic Fungi inNicotianaTabacum and Their Cytotoxicity
摘要
Abstract
To investigate the biodiversity, distribution, and the microbial community structure of the endophytic fungi inNicotiana tabacum, the endophytic fungi in different cultispecies were systematically isolated and identified. The preliminary screening of the cytotoxic activities was also performed. As a result, a total of 62 strains of the endophytic fungi were isolated and purified from the healthy tissues (roots, stems, and leaves) of tobacco using the tissue isolation method. The isolated strains were identified to belong to 4 orders, 7 families, and 9 genera based on their morphological and molecular characteristics. More species and strains were isolated from the leaves, followed by the stems, and those in the roots were the least. The cytotoxicity of the crude extracts was determined by CCK-8 assay. The results indicated that most of the strains were found to have some inhibitory activities on Caki-1 and HepG2 cell lines. The IC50s of the strain TE-6L to Caki-1 at 24 h and 48 h were 13.28 and 0.36μg/mL, respectively. Combined with morphological characteristics and molecular analysis, this strain was identified asFusarium sambucinum. The results from this study indicated that the tobacco possess abundant endophytic fungi, which provides a new path for searching for novel antitumor resources.关键词
烟草/内生真菌/种群分布/代谢产物/细胞毒活性Key words
tobacco/endophytic fungi/community distribution/secondary metabolites/cytotoxicity分类
农业科技引用本文复制引用
李盼盼,袁晓龙,郑璇,高林,刘新民,张忠锋,张鹏,申国明..烟草内生真菌多样性及其细胞毒活性初步研究[J].中国烟草科学,2017,38(3):74-79,6.基金项目
中国农业科学院科技创新工程(ASTIP-TRIC05) （ASTIP-TRIC05）
中国农业科学院烟草研究所青年科学基金项目"野生烟草 Nicotianaalata 化学成分多样性及抗植物病原菌活性研究"(2016A02) （2016A02）
