中国药房2017，Vol.28Issue(18)：2543-2545,3.DOI:10.6039/j.issn.1001-0408.2017.18.27
HPCE法测定金银花药材中木犀草苷、槲皮素和金丝桃苷的含量
Contents Determination of Luteoloside,Quercetin and Hyperoside in the Extract of Lonicera japonica by HPCE
摘要
Abstract
OBJECTIVE:To establish a method for contents determination of luteoloside,quercetin and hyperoside in Lonicera japonica. METHODS:HPCE was performed silica capillary column with detection wavelength of 360 nm and separation voltage of 20 kV,electrokinetic sampling,sampling voltage of 15 kV,sampling time of 5 s,operation temperature of 25 ℃.The buffer was consisted of 60 mmol/L sodium tetraborate-50 mmol/L natrium carbonicum-50 mmol/L hydroxypropyl-β-cyclodextrin(pH 9.2). RE-SULTS:The linear ranges of luteoloside,quercetin and hyperoside were 0.06-0.56mg/mL (r=0.9881),0.08-0.56 mg/mL (r=0.9892),0.06-0.49 mg/mL(r=0.9796),respectively. RSDs of precision,stability and reproducibility tests were all lower than 2.0%. The recoveries were 96.12%-99.77%(RSD=1.29%,n=6),95.90%-98.35%(RSD=0.89%,n=6),94.07%-97.45%(RSD=1.33%,n=6),respectively. CONCLUSIONS:The method is simple,accurate,stable and reproducible,and can be used for simultaneous determination of luteoloside,quercetin and hyperoside in L. japonica.关键词
高效毛细管电泳法/木犀草苷/槲皮素/金丝桃苷/金银花Key words
HPCE/Luteoloside/Quercetin/Hyperoside/Lonicera japonica分类
医药卫生引用本文复制引用
曾雪,杨元娟,陈竹,唐倩,石磊..HPCE法测定金银花药材中木犀草苷、槲皮素和金丝桃苷的含量[J].中国药房,2017,28(18):2543-2545,3.基金项目
重庆市基础与前沿研究计划项目(No.cstc2014jcy-jA10125) （No.cstc2014jcy-jA10125）
重庆医学检验试剂研究所科技创新项目(No.CQYJS1502) （No.CQYJS1502）
重庆市卫计委中医药科技项目(No.2012-2-17) （No.2012-2-17）
重庆市教委科学技术研究项目(No.KJ132501) （No.KJ132501）
