中国药房2017，Vol.28Issue(18)：2579-2581,3.DOI:10.6039/j.issn.1001-0408.2017.18.37
改进HPLC法测定酮洛芬肠溶胶囊中酮洛芬的含量
Content Determination of Ketoprofen in Ketoprofen Enteric-coated Capsules by Improving HPLC
郑旭霞 1周宇雪 2刘培建1
作者信息
- 1. 河南省洛阳正骨医院(河南省骨科医院)药剂科,郑州 450052
- 2. 郑州儿童医院药学部,郑州 450053
- 折叠
摘要
Abstract
OBJECTIVE:To improve HPLC for content determination of ketoprofen in Ketoprofen enteric-coated capsules. METHODS:HPLC method was adopted. The determination was performed on Chiralpak IC column with mobile phase consisted of n-hexane (0.1% TFA)-isopropanol(90:10,V/V)at a flow rate of 0.8 mL/min. The detection wavelength was set at 268 nm,and column temperature was 25 ℃. The sample size was 10 μL. RESULTS:The linear range of ketoprofen were 0.025-0.5 mg/mL(r=0.9998). The limit of quantitation was 1.0 mg/L,and limit of detection was 0.2 mg/L. RSDs of precision,stability and reproduc-ibility tests were lower than 2%;recoveries were 96.36%-100.32%(RSD=1.87%,n=6). CONCLUSIONS:The method is sim-ple,accurate and rapid,and can be used for the content determination of ketoprofen in Ketoprofen enteric-coated capsules.关键词
酮洛芬肠溶胶囊/酮洛芬/含量/高效液相色谱法Key words
Ketoprofen enteric-coated capsules/Ketoprofen/Content/HPLC分类
医药卫生引用本文复制引用
郑旭霞,周宇雪,刘培建..改进HPLC法测定酮洛芬肠溶胶囊中酮洛芬的含量[J].中国药房,2017,28(18):2579-2581,3.
