中国药理学通报2017，Vol.33Issue(7)：908-915,8.DOI:10.3969/j.issn.1001-1978.2017.07.006
骨形态蛋白9诱导干细胞骨向分化与环氧酶-2及PI3K/Akt的关系研究
Study on relationship between BMP9-induced osteogenicdifferentiation and COX-2/PI3K/Akt in stem cells
摘要
Abstract
Aim To investigated the possible effect of COX-2 on the BMP9-induced activation of PI3K/Akt signal in progenitor cells.Methods The activity of alkaline phosphatase(ALP) was measured using histochemical staining or chemiluminescence.The mRNA level of ALP was determined using real-time PCR assay.The protein levels of osteopontin(OPN), osteocalcin(OCN), COX-2, Akt1/2 and phosphorylated Akt1/2 were detected by Western blot.The mRNA level of COX-2 was assayed with RT-PCR, and the mineralization was measured with Alizarin Red staining.Results The ALP activity was apparently increased by BMP9 in C2C12 cells, as well as the protein level of OPN and OCN.The mineralization was also markedly induced by BMP9 in C2C12 cells.BMP9 increased the level of phosphorylated Akt1/2 greatly, although no substantial effect was observed on total protein level of Akt1/2.The BMP9-induced ALP activity was dramatically decreased by the inhibitor of PI3K.The mRNA and protein level of COX-2 were both increased by BMP9 in C2C12cells, and the BMP9-induced ALP activity and mineralization were greatly attenuated by the inhibitor of COX-2.The BMP9-induced phosphorylation level of Akt1/2 was increased by the exogenous expression of COX-2, but decreased by the inhibitor of COX-2.Conclusion Activation of PI3K/Akt signaling may be a critical event in BMP9-induced osteogenic differentiation, and this process may be mediated by the BMP9-upregulated COX-2 in stem cells at least.关键词
骨形态蛋白9/干细胞/成骨分化/成骨标志物/PI3K/Akt/环氧酶-2Key words
bone morphogenetic protein 9/stem cell/osteogenic differentiation/osteogenic marker/PI3K/Akt/cycloxygenase 2分类
医药卫生引用本文复制引用
李洋,陈前昭,邵英,曾于桦,任文艳,刘荣兴,何百成..骨形态蛋白9诱导干细胞骨向分化与环氧酶-2及PI3K/Akt的关系研究[J].中国药理学通报,2017,33(7):908-915,8.基金项目
国家自然科学基金资助项目(No 81372120) （No 81372120）
