中国药理学通报2017，Vol.33Issue(7)：915-921,7.DOI:10.3969/j.issn.1001-1978.2017.07.007
黄芪甲苷对甲基乙二醛诱导的人视网膜色素上皮细胞损伤的保护作用研究
Protective effect of astragalosides IV on retinal pigmentepithelium injury induced by methylglyoxal
摘要
Abstract
Aim To investigate the protective effect of astragaloside IV (AS-Ⅳ) on human retinal pigment epithelium injury induced by methylglyoxal (MGO), and explore its molecular mechanism.Methods The injury of ARPE-19 cells was induced by MGO and the cell viability was measured by CCK-8 method.The morphology of cell nucleus was analyzed by Hoechst 33342 staining and the cell apoptosis was analyzed by flow cytometry to detect labbled Annexin V-FITC/PI.JC-1 staining and fluorescence probe DCFH-DA were employed to evaluate the change of mitochondrial membrane potential and reactive oxygen species (ROS).The levels of SOD, MDA, caspase-9 and caspase-3 were determined by respective kits.Western blot was used to analyse the expression of Bcl-2, Bax and PARP.Results AS-Ⅳ could significantly inhibit the decrease of cell viability induced by MGO, improve the morphology of cell nucleus, reduce the ARPE-19 cell apoptosis rate and the level of ROS and MDA, and increase the activity of SOD.Furthermore, AS-Ⅳ could enhance mitochondrial membrane potential, the ratio of Bcl-2/Bax and the expression of PARP, and inhibit the activation of caspase-9 and caspase-3.Conclusion AS-Ⅳ may protect ARPE-19 cells from the injury induced by MGO by increasing the antioxidant ability of ARPE-19 cells and inhibiting cell apoptosis.关键词
糖尿病视网膜病变/黄芪甲苷/甲基乙二醛/人视网膜色素上皮细胞/抗氧化/细胞凋亡Key words
diabetic retinopathy/astragaloside IV/methylglyoxal/retinal pigment epithelium/antioxidation/apoptosis分类
医药卫生引用本文复制引用
周云丰,许扬,李琳,葛争艳,周立东,郭宇洁,金龙,任烨,李彦林,孙兰..黄芪甲苷对甲基乙二醛诱导的人视网膜色素上皮细胞损伤的保护作用研究[J].中国药理学通报,2017,33(7):915-921,7.基金项目
北京市自然科学基金资助项目(No 7152100) （No 7152100）
药植所创新团队发展计划(No IT1305) （No IT1305）
