中国药理学通报2017，Vol.33Issue(7)：922-927,6.DOI:10.3969/j.issn.1001-1978.2017.07.008
雪胆素甲诱导非小细胞肺癌系NCI-H460和A549的凋亡效应及其作用机制初探
Effects of cucurbitacin Ⅱa on apoptosis of humanlung cancer cell lines NCI-H460 and A549 and its mechanism
摘要
Abstract
Aim To study the apoptosis effect of cucurbitacin Ⅱa on non-small cell lung cancer cell lines NCI-H460 and A549 and its underlying mechanism.Methods Cell viability was assessed by CCK-8 assay.The apoptosis effect and cell cycle arrest were detected by Flow cytometry.Western blot was employed to detect the related protein.Results The proliferation of lung cancer cell lines NCI-H460 and A549 was inhibited by CuⅡa, which showed cytotoxic activity with IC50 values of 224.9 nmol·L-1 and 108.3 nmol·L-1 against NCI-H460 and A549 respectively.CuⅡa induced the cells apoptosis and cell cycle arrest at G2/M phase.The results of Western blot showed CuⅡa inhibited the phosphorylation of STAT3 and Cofilin in a dose-dependent manner.Further, CuⅡa inhibited the phosphorylation of Aurora A, in line with the important characteristics of anti-tumor effect of Aurora A kinase inhibitor with blocking cells in the G2/M phase.Conclusion CuⅡa has obvious anti-tumor effect against non-small cell lung cancer, which suggests its value as a lead compound for lung cell carcinoma.关键词
雪胆素甲/非小细胞肺癌/凋亡/AuroraA/Cofilin/STAT3Key words
curcerbitacin Ⅱa/non-small-cell lung cancer/apoptosis/Aurora A/Cofilin/STAT3分类
医药卫生引用本文复制引用
陈育林,刘进,张涛,阳泰,邹强,李华,咸婧,肖翠,钟月玲,苏小妹,徐丽,罗巧丽,程朋,王涛..雪胆素甲诱导非小细胞肺癌系NCI-H460和A549的凋亡效应及其作用机制初探[J].中国药理学通报,2017,33(7):922-927,6.基金项目
国家自然科学基金面上项目(No 81273530) （No 81273530）
成都军区十二五重大项目(No B14005) （No B14005）
医院研究型课题重大项目(No 2013YG-A004) （No 2013YG-A004）
