中国药理学通报2017，Vol.33Issue(7)：927-933,7.DOI:10.3969/j.issn.1001-1978.2017.07.009
肉苁蓉多糖对D-半乳糖所致急性衰老模型保护作用研究
In vivo and in vitro studies of protective effect of CDPS onacute aging mouse model induced by D-galactose
摘要
Abstract
Aim To study the protective effect of CDPS on acute aging mouse model induced by D-galactose (D-gal) and its mechanism.Methods (1) The acute aging mouse model was induced by D-gal.After CDPS (25、50、100 mg·kg-1) treatment, the improving effect on learning and memory in mice was examined in vivo.(2) We also established the aging model on PC12 cells in vitro.After CDPS treatment (150、200 mg·L-1), the level of p-CREB in the nucleus was detected by Western blot, and the content of cAMP, PKA and brain derived neurotrophic factor (BDNF) levels were examined by the Elisa kits.Moreover, cAMP, PKA and BDNF were detected in PC12 cells under the condition that H89, the inhibitor of PKA, co-cultured with PC12 cells after CDPS treatment.(3) The UPLC/Q Exactive MS method was developed for determining the concentration of glutamic acid, dopamine and norepinephrine, which secreted in PC12 cells after CDPS treatment.Results (1) In vivo, CDPS significantly improved the memory impairment in aging mice induced by D-gal in the Morris assay.(2) In vitro, CDPS could significantly increase the expression of p-CREB (P<0.05), PKA, cAMP and BDNF (P<0.05).The H-89 abolished the increase of p-CREB (P<0.05), PKA, cAMP and BDNF (P<0.05) in PC12 aging cells induced by D-gal after CDPS treatment.(3) CDPS increased the release of dopamine, norepinephrine, and glutamate secreted in PC12 cells.Conclusion CDPS could significantly improve the learning and memory ability on aging mouse model in vivo, and reversed the damage in PC12 cells induced by D-gal by activating cAMP/PKA/CREB signal cascade, increase the expression of BDNF, and increasing modestly the release of excitatory neurotransmitter.关键词
肉苁蓉多糖/cAMP/PKA/CREB/BDNF/神经递质/抗衰老/D-半乳糖Key words
CDPS/CAMP/PKA/CREB/BDNF/Neurotransmitters/anti-aging/D-galactose分类
医药卫生引用本文复制引用
武燕,张弘,布仁,马慧,苏苗,李刚..肉苁蓉多糖对D-半乳糖所致急性衰老模型保护作用研究[J].中国药理学通报,2017,33(7):927-933,7.基金项目
国家自然科学基金资助项目(No 81260650) （No 81260650）
内蒙古科技厅项目(No KJT14bs805) （No KJT14bs805）
内蒙古教育厅资助项目(No JYT141133、JYT14qnyco3) （No JYT141133、JYT14qnyco3）
