中国药理学通报2017，Vol.33Issue(7)：1026-1031,6.DOI:10.3969/j.issn.1001-1978.2017.07.027
荞麦花叶发酵提取物对2型糖尿病db/db小鼠心肌损伤的影响
Effects of extract from fermented buckwheat flower and leaf(EFBFL)on myocardial injury in type 2 diabetic db/db mice
摘要
Abstract
Aim To discuss the effectsof extract from fermented buckwheat flower and leaf(EFBFL) on myocardial injury in spontaneously obese type Ⅱ diabetic db/db mice and its mechanism.Methods 9-week-old male db/db mice were randomly divided into high level EFBFL dose group(EFBFL-H, 0.1 g·kg-1), low level EFBFL dose group(EFBFL-L, 0.05 g·kg-1),metformin hydrochloridecontrol group, model control group, and normal control group, with 10 mice in each group.All groups were treated with 8 wks of drugs by gastric perfusion.The random blood glucose(RBG) was tested respectively at the end of 2nd, 4th, 6th, and 8th wk.Finally, the levels of creatine kinase(CK) creatine kinase MB(CK-MB), andadvanced glycosylation endproducts(AGEs) were detected after 8 wks.The morphological changes of myocardium were observed under light microscope by HE staining, and the ultrastructure of myocardium was observed under electron microscope.Immunohistochemical method and Western blot were used to detect myocardial tissue glucose transporter-4(Glut-4).Results EFBFLcould repress patho-proceeding of myocardial fibrosis efficiently, and significantly decrease the level of blood glucose, CK,CK-MB, and AGEs in db/db mice.Meanwhile, it could increase the expression of Glut-4 in myocardial tissues of mice.Conclusions EFBFL can prevent myocardial injury in spontaneously obese type Ⅱ diabetic db/db mice.The possible mechanism may be related to lowering the level of blood glucose and serum AGEs and up-regulating Glut4of cardiac muscle.关键词
荞麦花叶发酵提取物/2型糖尿病/糖尿病性心脏病/心肌损伤/db/db小鼠/葡萄糖转运蛋白4Key words
extract from fermented buckwheat flower and leaf/type 2 diabetes mellitus/diabetic cardiopathy/myocardial injury/db/db mice/Glut-4分类
医药卫生引用本文复制引用
王建行,王妍,刘曼,穆玉,张博,韩淑英..荞麦花叶发酵提取物对2型糖尿病db/db小鼠心肌损伤的影响[J].中国药理学通报,2017,33(7):1026-1031,6.基金项目
河北省自然科学基金资助项目(No C2010001795) （No C2010001795）
河北省中医药管理局科研计划项目(No 2017083) （No 2017083）
