中国药业2017，Vol.26Issue(11)：9-12,4.DOI:10.3969/j.issn.1006-4931.2017.11.003
清湿外洗汤质量控制研究
Quality Control of Qingshiwaixi Decoction
摘要
Abstract
Objective To establish a quality control system of Qingshiwaixi Decoction extracted by two fried normal pressure decocting machine. Methods The main ingredients of Fructus Gardeniae, Cortex Phellodendri, Fructus Cnidii in Qingshiwaixi Decoction were identified by TLC, the content of main active ingredient matrine in Qingshiwaixi Decoction was determined by HPLC with XTerra Rp18 column ( 250 mm × 4. 6 mm, 5 μm ) , acetonitrile-ethylenediamine-water ( 45 :0. 05 :55 ) was used as mobile phase, the flow rate was 1. 0 mL/min, the column temperature was set at 35 ℃, the wavelength of the detector was set at 220 nm. The content change of ma-trine was observed within 7 days at room temperature and avoided light, meanwhile, microbial limit test was carried out. Results The TLC method had no negative interference, good separation and highly specificity with all the spots were clear. The linear range for ma-trine was 0. 0295-0. 177 g/L ( r=0. 9998 ) . The average recovery were 97. 41%( n=6 ) . The content of matrine in Qingshiwaixi De-coction showed 1. 85% decrease in the 7th day. Conclusion The method is simple, highly sensitive, highly repeatable and specific, it can be used for quality control of Qingshiwaixi Decoction. Qingshiwaixi Decoction can be kept stably at room temperature and avoided light lasting 7 days after it was extracted by two fried normal pressure decocting machine.关键词
清湿外洗汤/苦参碱/薄层色谱法/高效液相色谱法/质量控制Key words
Qingshiwaixi Decoction/matrine/TLC/HPLC/quality control分类
医药卫生引用本文复制引用
薛敏菲,雷凯君,吴舜芳,黄建明..清湿外洗汤质量控制研究[J].中国药业,2017,26(11):9-12,4.基金项目
广东省佛山市医学类科技攻关项目[ 2016AB002051 ]. （）
