中国药业2017，Vol.26Issue(11)：25-27,3.DOI:10.3969/j.issn.1006-4931.2017.11.008
高效液相色谱法同时测定健脾益肾方水提物中松果菊苷、丹酚酸B和大黄酸含量
Simultaneously Content Determination of Echinacoside, Salvianolic Acid B and Rheic Acid in Jianpi Yishen Formula by HPLC
摘要
Abstract
Objective To establish an HPLC method for the content determination of echinacoside, salvianolic acid B and rheic acid in Jianpi Yishen Formual simultaneously. Methods The Inertsil ODS-SP column ( 150 mm × 4. 6 mm, 5 μm ) was adopted with acetoni-trile-0. 2% phosphoric acid as mobile phase using gradient program, the flowing rate was 1. 0 mL/min, the detection wavelength was 330 nm ( echinacea ) , 286 nm ( salvianolic acid B ) , and 254 nm ( rhein acid ) , the sample size was 10 μL. Results The calibration curves of echinacoside, salvianolic acid B and rheic acid were linear from 2. 50-80. 00 μg/mL ( r=0. 9994 ) , 1. 25-80. 00 μg/mL ( r=0. 9993 ) , 1. 25-40. 00 ( r=0. 9994 )μg/mL, the accuracy, stability, and reproducibility of the RSD were less than 2. 00% ( n=6 ) , the average recovery was 100. 01%, 99. 15%, 98. 95%, respectively, the RSD was less than 2. 00% ( n=6 ) . Conclusion The method is simple, efficient, accurate and reproducible, which can be used for the content determination of echinacoside, salvianolic acid B and rheic acid in Jianpi Yishen Formula.关键词
健脾益肾方/高效液相色谱法/含量/松果菊苷/丹酚酸B/大黄酸Key words
Jianpi Yishen Formula/HPLC/content/echinacoside/salvianolic acid B/rheic acid分类
医药卫生引用本文复制引用
何泰东,余晓丹,郑平,邹美南,张尚斌,陈剑平,李顺民..高效液相色谱法同时测定健脾益肾方水提物中松果菊苷、丹酚酸B和大黄酸含量[J].中国药业,2017,26(11):25-27,3.基金项目
广东省自然科学基金项目[ 2015 A 030310247 ] （）
广东省中医药局项目[ 20162124 ] （）
广东省深圳市科技计划项目[JSGG20141017103353178,JCYJ20150401163247213,ZDSYS20160608151545865 ]. （）
