中国药业2017，Vol.26Issue(12)：11-14,4.DOI:10.3969/j.issn.1006-4931.2017.12.003
五味子乙素调控NF-κB通路对顺铂致HK-2细胞炎症损伤的保护作用
Protective Effect of Schisandrin B for Protecting Cisplatin-Induced Inflammatory Damage of HK-2 Cells Through Regulating NF-κB Pathway
摘要
Abstract
Objective To investigate the protective effect of schisandrin B against Cisplatin-induced inflammation damage in HK-2 cells and its related mechanism. Methods After treated with various concentrations of schisandrin B and Cisplatin,viability of HK-2 cells was determined by CCK-8 assay;cellular morphology was evaluated by Hoechst 33258 staining;activation of NF-κB was detect-ed by Western blot;levels of TNF-α,IL-1β,IL-6 in supernatants were measured by ELISA,mRNA expressions of TNF-α,IL-1β, IL-6 were detected by RT-PCR. Results Compared with the control group,the cell activity of the cisplatin group decreased signifi-cantly,and the cell morphology changed significantly. Schisandrin B dose-dependently reduced the cytotoxicity induced by cisplatin,at the same time,schisandrin B significantly inhibited the activation of NF-κB induced by Cisplatin and reversed the increase of levels of TNF-α,IL-1β,IL-6 in supernatants and mRNA expressions. Conclusion Schisandrin B can against Cisplatin-induced inflammation damage in HK-2 cells through regulating NF-κB pathway.关键词
五味子乙素/顺铂/肾毒性/炎性反应/NF-κB通路/HK-2细胞Key words
schisandrin B/Cisplatin/nephrotoxicity/inflammatory reaction/NF-κB pathway/HK-2 cell分类
医药卫生引用本文复制引用
李佳,尹一子,简晓顺,林蔚..五味子乙素调控NF-κB通路对顺铂致HK-2细胞炎症损伤的保护作用[J].中国药业,2017,26(12):11-14,4.基金项目
广州医科大学博士启动项目[ 2013C64 ]. （）
