中国药业2017，Vol.26Issue(12)：23-26,4.DOI:10.3969/j.issn.1006-4931.2017.12.006
香樟果挥发性成分不同提取方法的气相色谱-质谱联用分析及其对神经细胞活性的影响
Volatile Oils of Fruits of Cinnamomum Camphora with Different Extraction Methods by GC-MS and Its Effect on Nerve Cell Activity
杨锦强 1杨念云 1于生 1崔小兵 1郭舒臣1
作者信息
摘要
Abstract
Objective To analyze and compare the methods of solid-phase microextraction(SPME)and steam distillation for extracting volatile oils from fruits of Cinnamomum Camphora by GC-MS and to study its effect on nerve cell activity. Methods The volatile oils were extracted by SPME and steam distillation,analyzed and identified by GC-MS. The compounds were quantitatively determined by normalization method. The effect of volatile oils extracted by steam distillation on nerve cell activity was study by contrast method. Results The main volatile compounds extracted from fruits of Cinnamomum Camphora by the 2 methods were different. Totally 26 compounds were identified by SPME from fruits of Cinnamomum Camphora. The major chemical constituents were β-thujene (16. 07%),camphor(14. 31%),3-carene(13. 70%),eucalyptol(11. 53%)and camphene(5. 09%). Totally 23 compounds were identified by steam distillation from fruits of Cinnamomum Camphora. The major chemical constituents were safrole(19. 81%),eucalyptol(9. 68%) and linalool(5. 88%). The volatile oils at the concentration of 100 μg/mL could enhance the activity of nerve cells. Conclusion The main volatile compounds extracted from fruits of Cinnamomum Camphora by the 2 methods were different,different extraction methods can be adopted according to different target products. Meanwhile,the extraction of volatile components of Cinnamomum Camphora by SPME is a combination of extraction and sample introduction. It has simple operation,good repeatability,and practical value for the quality control of Cinnamomum Camphora.关键词
香樟果/挥发性成分/气相色谱-质谱联用法/固相微波萃取/水蒸气蒸馏/神经细胞活性Key words
Cinnamomum Camphora/volatile oil/GC-MS/SPME/steam distillation/nerve cell activity分类
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杨锦强,杨念云,于生,崔小兵,郭舒臣..香樟果挥发性成分不同提取方法的气相色谱-质谱联用分析及其对神经细胞活性的影响[J].中国药业,2017,26(12):23-26,4.
