中华临床免疫和变态反应杂志2017，Vol.11Issue(2)：142-148,7.DOI:10.3969/j.issn.1673-8705.2017.02.008
镍变应性接触性皮炎小鼠动物模型建立
Establishment of A Mouse Model of Nickel-Induced Allergic Contact Dermatitis
摘要
Abstract
Objective To establish a nickel-induced allergic contact dermatitis (ACD) mouse model.Methods Thirty C57BL/6 mice were divided into 5 groups based on the concentration of nickel sulfate (5%-5%, 10%-10%, 10% single administration, positive control and negative control).All mice were sensitized at day 0 and challenged at day 2 and day 3.Grading of the skin alterations was performed at 6, 12, 24, and 48 h after first challenge respectively.The skin thickness of mice was detected by ultrasonic scan.Pathological changes were evaluated by hematoxylin and eosin stain (HE) staining among cutaneous biopsy samples.IFN-γ, IL-4, and IL-10 levels were detected in skin homogenates 48 h after challenge.Results The severest inflammatory reactions were observed at 24 h in all nickel sulfate challenge groups and epidermis incrassation were observed in all nickel sulfate challenge groups.The thickness of epidermis based on the results of ultrasonic scan were as follow: 10%-10%>5%-5%>10% single challenge group.Acute inflammatory reaction such as escharosis on epidermis, liquefaction of stratum basalis, angiotelectasis and inflammatory cell infiltration in dermis was observed in 5%-5% and 10%-10% group, which is similar to pathological feature of acute phase of human ACD.The cutaneous levels of interferon-γ, interleukin-2, interleukin-10 of 5%-5% and 10%-10% group are higher than negative control group, the differences were significant statistically (P<0.05).Conclusion The mouse models of nickel-induced ACD had been established successfully in 5%-5% and 10%-10% group.关键词
硫酸镍/变应性接触性皮炎/动物模型/小鼠Key words
nickel sulfate/allergic contact dermatitis/animal model/mouse分类
医药卫生引用本文复制引用
魏若尧,孙井江,李挚,刘跃华,高虹,孙劲旅..镍变应性接触性皮炎小鼠动物模型建立[J].中华临床免疫和变态反应杂志,2017,11(2):142-148,7.基金项目
国家自然科学基金(81373175),中国医学科学院医学与健康科技创新工程项目(2016-12M-1-003) （81373175）
