中华临床免疫和变态反应杂志2017，Vol.11Issue(2)：149-155,7.DOI:10.3969/j.issn.1673-8705.2017.02.009
鸡蛋组分蛋白对临床诊断儿童鸡蛋过敏的价值
Evaluation of Hen's Egg Protein Components for Clinical Diagnosis of Hen's eggAllergy among Children
张书辰 1王晓龙 1祝戎飞 1杨林 1张威 1李文静 1黄南 1项宁 1汪茵 1胡林涛1
作者信息
- 1. 华中科技大学同济医学院附属同济医院过敏反应科,武汉430030
- 折叠
摘要
Abstract
Objective To investigate the immunogenicity of synthetic hen's egg protein components (Gal d1, Gal d2, Gal d3 and Gal d4) by using plasmid transduction, and the correlations among hen's egg protein components, specific IgE (f1) and the skin test results among hen's egg-allergic patients.Methods Hen's egg protein components were synthesized by using plasmid transduction, whereas the immunogenicity of the target proteins was analyzes based on Western blot.The clinical diagnostic values of each protein component were evaluated, based on the binding of protein components with specific IgE of hen's egg-allergic patients.Results Immunogenicity was shown successfully among synthetic hen's egg protein components (Gal d1, Gal d2, Gal d3 and Gal d4).The positive rate of skin prick test (SPT) of patients whose hen's egg specific IgE (f1)>10 kU/L was significantly higher than of patients whose specific IgE (f1)<10 kU/L (P=0.000 3).Ovomucin (Gal d1, D450 nm=0.81) and ovalbumin (Gal d2, D450 nm=0.567) are the major protein components of hen's egg allergen.Ovalbumin (Gal d2) specific IgE level was higher among patients whose hen's egg specific IgE (f1)>10 kU/L, whereas ovomucin (Gal d1) specific IgE level higher among patients whose hen's egg specific IgE (f1)>10 kU/L.Conclusion Immunogenicity was shown successfully among synthetic hen's egg protein components (Gal d1, Gal d2, Gal d3 and Gal d4), which should be of value for the purification of food allergens, and also of importance in the diagnosis of hen's egg allergy.关键词
蛋类过敏/免疫球蛋白E/皮肤试验/组分蛋白Key words
hen's egg allergy/immunoglobulin E/skin tests/protein components分类
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张书辰,王晓龙,祝戎飞,杨林,张威,李文静,黄南,项宁,汪茵,胡林涛..鸡蛋组分蛋白对临床诊断儿童鸡蛋过敏的价值[J].中华临床免疫和变态反应杂志,2017,11(2):149-155,7.
