针灸临床杂志2017，Vol.33Issue(5)：60-64,89,6.
夹脊电针对ALS-SOD1G93A转基因小鼠腰髓前角运动神经元形态学的影响
Effect of Electroacupuncture at Jiaji Points(EX-B2) on Morphology of Lumbar Spinal Anterior Horn Neurons in the Transgenic Mice with Amyotrophic Lateral Sclerosis (ALS)
摘要
Abstract
Objective:To observe the effect of electroacupuncture at Jiaji point (EX-B2) on morphology of lumbar spinal anterior horn neurons in the transgenic mice with ALS.Methods:24 ALS-SOD1 G93A transgenic mice were randomly divided into three even groups,that is electroacupuncture (group E),acupuncture group (group A) and model group (group M).Eight non-transgenic littermates were assigned to blank group (group B).Interventions to ALS-SOD1G93A transgenic mice began from their age of 60 days.Group E was treated by electroacupuncture at Jiaji points (EX-B2),with the electrical stimulation at the frequency of 2 Hz.The four-week intervention was 20 minutes per time and twice a week.Group A was only treated by acupuncture at Jiaji points (EX-B2) and group C was disposed by firm binding.None intervention was given to group B.At the age of 120 days,HE staining,Nissl staining and transmission electron microscopy were used to ALS-SOD1 G93A transgenic mice to observe the anterior horn of the lumbar spinal cord pathological morphology,and detect the amount of motor neurons.Results:Analysis from HE staining,Nissl staining and transmission electron microscopy showed that group E and group A could significantly reduce morphological lesions and increase the amount of motor neurons compared with group C (P ＜ 0.05 or P ＜ 0.01),and group E was better than group C.Conclusion:Electroacupuncture at Jiaji points(EX-B2) can improve morphological changes of motor neurons in the anterior horn of the lumbar spinal cord in ALS-SOD1G93A transgenic mice,which is better than by manual acupuncture.关键词
夹脊/电针/ALS-SOD1 G93A/尼氏染色/电镜Key words
Jiaji points (EX-B2)/Electroacupuncture/AlS-SOD1 G93A/Nissl staining/Electron microscopy分类
医药卫生引用本文复制引用
郭颖,祝鹏宇,孙颖哲,孙远征,赵广然,武丹,刘彦麟..夹脊电针对ALS-SOD1G93A转基因小鼠腰髓前角运动神经元形态学的影响[J].针灸临床杂志,2017,33(5):60-64,89,6.基金项目
国家中医药管理局全国名老中医药专家传承工作室建设项目,编号:国中医药人教发[2014]20号. （）
