针灸临床杂志2017，Vol.33Issue(5)：65-69,5.
针康法对脑缺血大鼠脑组织微血管内皮超微结构及aFGF、PF4蛋白表达影响
Influence of Acupuncture-Rehabilitation Therapy on Microvascular Endothelial Ultrastructure, Expression of aFGF and PF4 in Cerebral Ischemia Rats
摘要
Abstract
Objective:To investigate the influence of Acupuncture-Rehabilitation Therapy on microvascular endothelial ultrastructure and expression of acidic fibroblast growth factor (aFGF) and platelet factor-4 (PF4)in cerebral ischemia rats.Methods:Permanent focal cerebral ischemia was established in rats according to Longa methods.180 rats were randomly divided into model group (group M),sham group (group C),acupuncture group(group A),rehabilitation group (group R) and acupuncture combined with rehabilitation group (group A-R).No treatment was given to group M and group C.Cluster needling of scalp acupuncture was given to group A.Treadmill training was used for group R.The combination therapy of acupuncture and treadmill training was used for group A-R.Ultrastructure of microvascular endothelial around penumbral cortex area,facilitated factor aFGF and inhibitory factor PF4 were observed and detected by electron microscopy at each time point by Western Blot method.Results:Microvessel endothelia ultrastructure:3 days after modeling,endotheliocytes were filled in lumen and edema around was relieved in group A,group R and group A-R,of which group A-R was better than the other two groups.7 days after modeling,the proliferation of endotheliocytes was significant,microvascular endangium was smooth and no stricture developed in lumen in group A-R;Unsmooth microvascular endangium was found in the other two groups.14 days after modeling,microvascular basement membrane was complete and endotheliocytes returned to almost normal in group A-R;Microvascular basement membrane was a bit incomplete both in group A and group R.Expression of revascularization facilitated factor aFGF and inhibitory factor PF4:At various time points,compared with group M,the expression of aFGF was all higher (P ＜ 0.05) and the expression of PF4 was all lower (P ＜ 0.05) in the three intervened groups.7 days and 14 days after modeling,the expression of aFGF in group A-R was obviously higher (P ＜ 0.05) and the expression of PF4 protein was obviously lower (P ＜ 0.05) than those in group A and group R.Conclusion:Acupuncture-Rehabilitation Theray can reduce the vascular endothelial cell injury,which may be associated with the up-regulation of aFGF protein expression and down-regulation of PF4 protein expression.关键词
脑缺血/针康法/酸性成纤维细胞生长因子/血小板第4因子/血管新生Key words
Cerebral ischemia/Acupuncture-rehabilitation Therapy/Acidic fibroblast growth factor/Platelet factor-4/Angiogenesis分类
医药卫生引用本文复制引用
陈慧杰,唐强,朱路文,叶涛,李季,颜培宇,王艳..针康法对脑缺血大鼠脑组织微血管内皮超微结构及aFGF、PF4蛋白表达影响[J].针灸临床杂志,2017,33(5):65-69,5.基金项目
国家自然科学基金项目,编号:81273818,81473762 （）
黑龙江中医药大学领军人才计划项目,编号:2012RCL02. （）
