浙江农林大学学报2017，Vol.34Issue(3)：552-558,7.DOI:10.11833/j.issn.2095-0756.2017.03.022
蔺草种质资源遗传多样性的ISSR分析
Genetic diversity of Juncus effusus germplasm by ISSR markers
摘要
Abstract
To study the genetic diversity of Juncus effusus res ources, the genetic diversity of 36 J. effusus germplasm resources from both home and abroad were analyzed by Inter Simple Sequence Repeats (ISSR) markers. Analysis included an Unweighted Pair Group Method with Arithmetic Mean (UPGMA) cluster analy-sis. Results showed 23 primers with clear polymorphic bands selected from 72 primers. A total of 148 bands, of which 130 were polymorphic, were amplified from the 36 materials with the proportion of polymorphic loci be-ing 87.84%. The genetic similarity coefficient for varieties was between 0.4056 and 0.9441, which showed rich genetic diversity. The UPGMA cluster analysis produced a genetic similarity coefficient of 0.6503 for the boundaries with the 36 materials divided into five groups and the first group being divided into three sub-groups. Results were connected to region and cultivar origin with materials from the same region mostly classi-fied into the same group or subgroup. This research revealed the genetic diversity of J. effusus germplasm re-sources at the molecular level for the first time which could provide a theoretical basis for breeding.关键词
植物学/蔺草/种质资源/ISSR标记/遗传多样性Key words
botany/Juncus effusus/germplasm resources/ISSR markers/genetic diversity分类
农业科技引用本文复制引用
柴晓娟,郭玮龙,陈慧,王俊荣,谢德志,金水虎..蔺草种质资源遗传多样性的ISSR分析[J].浙江农林大学学报,2017,34(3):552-558,7.基金项目
浙江省宁波市科技资助项目(2012C10039) （2012C10039）
