浙江医学2017，Vol.39Issue(12)：946-951,6.DOI:10.12056/j.issn.1006-2785.2017.39.12.2016-2210
茶多酚对人前列腺癌PC-3M细胞侵袭力的影响
Effect of tea polyphenols on invasive ability of prostate cancer PC-3M cells
严正强 1陈铁定 1陈力 1张琴 1杨远清1
作者信息
- 1. 315103 宁波市医疗中心李惠利东部医院泌尿外科
- 折叠
摘要
Abstract
Objective To investigate the effect of tea polyphenols (TPs) on invasive ability of human prostate cancer PC-3M cells.Methods Human prostate cancer PC-3M cells were treated with 40,60,80μg/ml tea polyphenol for 24h or 48h in vitro.The invasive ability of PC-3M cells was determined by Transwell assay,and the expression of MMP-2,TIMP-2 mRNA and protein was detected by RT-PCR and Western blot,respectively.Results Transwell assay showed that the invasive ability of PC-3M cells was decreased after treatment with tea polyphenol.The expression of MMP-2 mRNA and protein in PC-3M cells was increased after treated with 60μg/ml and 80μg/ml for 24h or 48h were significantly decreased (P＜0.05),while the expression of TIMP-2 mRNA and protein was increased (P＜0.05) in a dose-dependent and time-dependent manner (P＜0.05).Conclusion Tea polyphenols can reduce the invasive ability of human prostate cancer PC-3M cells in vitro,which may be related to the down-regulation of MMP-2 and up-regulation of TIMP-2 gene expression.关键词
茶多酚/前列腺癌/肿瘤侵袭/基质金属蛋白酶-2/金属蛋白酶组织抑制因子-2Key words
Tea polyphenol/Prostate cancer/Neoplasm invasiveness/MMP-2/TIMP-2引用本文复制引用
严正强,陈铁定,陈力,张琴,杨远清..茶多酚对人前列腺癌PC-3M细胞侵袭力的影响[J].浙江医学,2017,39(12):946-951,6.
