浙江医学2017，Vol.39Issue(12)：979-983,989,6.DOI:10.12056/j.issn.1006-2785.2017.39.12.2017-470
microRNA-616在肝细胞癌侵袭转移中的作用机制研究
Expression of miR-616 in human hepatocellular carcinoma and its effect on tumor invasion and metastasis
摘要
Abstract
Objective To investigate the expression of miR-616 in human hepatocellular carcinoma and its effects on tumor invasion and metastasis.Methods The expression of miR-616 was detected with real-time quantitative PCR (qRT-PCR) in 60 specimens of hepatocellular carcinoma (HCC) tissues and corresponding adjacent'normal tissues;and also in human HCC HepG2,SMMC-7721,Hep3B,Hu75 cells and normal liver LO2 cells.The expression of vimentin,phosphatase,tensin homolog (PTEN) and E-cadherin was detected with immunohistochemical S-P method.The miR-616 inhibitor was transfected into Hep3B cells in vitro,cell invasion was analyzed by Transwell? assay.The expression of PTEN,E-cardhern and vimentin mRNAs and proteins after transfection was detected by qRT-PCR and Western blotting,respectively.Results The expression of miR-616 in HCC tissues was higher than that in matched tumor-adjacent normal tissues;and it was significantly correlated with venous infiltration,high Edmondson-Steiner grades and lymph node metastasis and TNM tumor stages in HCC.Elevated miR-616 expression was observed in HCC cell lines HepG2,SMMC-7721,Huh7 and Hep3B as compared with that in LO2 hepatic cell line.Furthermore,miR-616 expression was negatively correlated with E-cadherin and PTEN,positively correlated with vimentin in HCC tissues.The miR-616 inhibitor suppressed the migration and invasion in Hep3B cells;and it increased the expression of E-cadherin and PTEN,while decreased the expression of vimentin in Hep3B cells.In addition,down-regulation of PTEN'expression partially abrogated the effect of miR-616 inhibitor in HCC cells.Conclusion The expression of miR-616 in HCC tissues is significantly up-regulated,and it can promote proliferation and induce apoptosis of HCC cells by down-regulating the expressions of PTEN.关键词
miR-616/肝细胞癌/侵袭/靶基因同源性磷酸酶-张力蛋白/上皮间质转化Key words
mcroRNA-616/Hepatocellular carcinoma/Migration and invasion/Phosphatase and tensin homolog(PTEN)/Epithelial-mesenchymal transition引用本文复制引用
刘欣,许秋然,张美齐,蔡文伟,刘青光,涂建锋..microRNA-616在肝细胞癌侵袭转移中的作用机制研究[J].浙江医学,2017,39(12):979-983,989,6.基金项目
浙江省自然科学基金(LY16H160043) （LY16H160043）
浙江省医药卫生科技计划一般项目(2016KYA022、2015KYB033) （2016KYA022、2015KYB033）
