浙江医学2017，Vol.39Issue(12)：1005-1008,后插2,5.DOI:10.12056/j.issn.1006-2785.2017.39.12.2016-2015
miR-630在肺腺癌组织中的表达及其对肺腺癌细胞迁移侵袭的影响
Expression of miR-630 in lung adenocarcinoma and its effect on tumor migration and invasion
摘要
Abstract
Objective To investigate the expression of miR-630 in lung adenocarcinoma and its effect on tumor invasion and metastasis.Methods The expressions of miR-630 and SOX4 mRNA were detected by RT-PCR in 37 samples of lung adenocarcinoma tissue and matched normal lung tissue.Pearson correlation analysis was performed to test the correlation of miR-630 with SOX4 mRNA.Human lung adenocarcinoma A549 cells were transfected with miR-630.The migratory ability of A549 cells was tested by wound healing and Transwell assays;the expressions of COL1A1,COL1A5 and SOX4 in A549 cells were detected by Wester n blotting;Luciferase assay was used to confirmed whether SOX4-3'-UTR was the target gene of miR-630.Results Compared with normal lung tissue,the expression of miR-630 was decreased and SOX4 mRNA was significantly increased in lung adenocarcinoma (P＜0.05,P＜0.01).Pearson correlation analysis showed that miR-630 was negatively correlated with SOX4 mRNA (r=-0.344,P＜0.01).In vitro experiment,compared with the wild A549 cells,the expressions of COL1A1,COL1A5 and SOX4 were decreased in the miR-630-transfected A549 cells (P＜0.01).The migration activity of A549 cells was decreased after transfected with miR-630 (P＜0.01).The Luciferase activity of the SOX4-3'-UTR plasmid was significantly suppressed by miR-630 (P＜0.00).Conclusion The expression of miR-630 is down-regulated in lung adenocarcinoma;miR-630 inhibits migration and invasion of A594 cells,indicating that SOX4-3'-UTR may be its targeting gene.关键词
肺腺癌/微小RNA-630/SOX4/侵袭Key words
Lung adenocarcinoma/miR-630/Sex determining region Y-box 4/Invasion引用本文复制引用
谢铭芳,唐蒙蒙,丁伟..miR-630在肺腺癌组织中的表达及其对肺腺癌细胞迁移侵袭的影响[J].浙江医学,2017,39(12):1005-1008,后插2,5.基金项目
浙江省自然科学基金项目(LY14H0200002) （LY14H0200002）
