制冷学报2017，Vol.38Issue(3)：114-118,5.DOI:10.3969/j.issn.0253-4339.2017.03.114
低温保护剂添加与去除过程对猪卵母细胞的渗透损伤
Osmotic Injury to Porcine Oocytes during Cryoprotective Agents Addition and Removal Processes
摘要
Abstract
Oocyte cryopreservation has become a practical tool in assisted reproductive technology and fertility preservation. Cryoprotec-tive agents ( CPAs) addition and removal are essential steps during oocytes cryopreservation, however, the processes of CPAs addition and removal may cause fatal osmotic and toxic injuries to oocytes. In order to study the injuries of porcine MⅡ-stage oocytes during CPAs addi-tion and removal combined processes and minimize osmotic and toxic injuries to oocytes, primarily, a microfluidic device for continuous loading and unloading of CPAs was designed and fabricated in this study. CPAs were loaded and unloaded with the microfluidic linear method and the conventional step-wise method, respectively. Then, the cell volume changes and the effects on the survival rate and devel-opmental potential of oocytes were investigated. The results showed that the oocyte volume changes with the microfluidic device were obvi-ously less than step-wise method. The survival, cleavage and blastocyst rate of oocytes were 95. 3%, 64. 4%, and 19. 4%, respectively, which were significantly higher than the traditional step-wise method ( 79. 4%, 43. 6%, and 9. 7%) ( P<0. 05 ) . In conclusion, mi-crofluidic device can be used efficiently to load and unload of CPAs, and significantly reduce the osmotic shock to oocytes, which may open up a new path for oocyte cryopreservation.关键词
低温保存/低温保护剂/渗透损伤/卵母细胞Key words
cryopreservation/cryoprotective agents( CPAs)/osmotic injury/oocyte分类
通用工业技术引用本文复制引用
杨云,周新丽,戴建军,张德福,衣星越,滕芸,陶乐仁..低温保护剂添加与去除过程对猪卵母细胞的渗透损伤[J].制冷学报,2017,38(3):114-118,5.基金项目
国家自然科学基金( 51376132 )资助项目. ( The project was supported by the National Natural Science Foundation of China ( No. 51376132). )本文受上海市国家重点实验室(1N15301101)项目资助.( The project was supported by the National Key Laboratory of Shanghai (No. 1N15301101). ) （ 51376132 ）
