中南民族大学学报（自然科学版）2017，Vol.36Issue(2)：25-29,5.
OsmC基因在豌豆根瘤菌抗氧化和共生中的功能
Antioxidative and Symbiosis Function of OsmC gene in Rhizobium leguminosarum
摘要
Abstract
To study the antioxidative and symbiosis nitrogen fixation function of OsmC gene in Rhizobium leguminosarum, OsmC mutant RLOsmC was constructed by homologous recombination.The results indicated that mutation of OsmC gene had no effect on the self-growth ability of Rhizobium leguminosarum, but it was significantly sensitive to both H2O2 and cumene hydroperoxyde (CuOOH) with low concention.Red effective nodules could be formed when mutant strains RLOsmC infected host peas, but the capacity of nitrogenase was reduced by 18.3%.OsmC gene expression could not be induced by H2O2, while its expression was markedly increased in 7 d nodule bacteroids.Overall, it was revealed that rhizobial OsmC gene played an important role in symbiotic and antioxidative function.关键词
豌豆根瘤菌/OsmC基因/抗氧化功能/共生功能/实时荧光定量RT-PCRKey words
Rhizobium leguminosarum/OsmC gene/antioxidative function/symbiosis function/real-time RT-PCR分类
生物科学引用本文复制引用
程国军,彭杨,殷杰,田梦洋..OsmC基因在豌豆根瘤菌抗氧化和共生中的功能[J].中南民族大学学报（自然科学版）,2017,36(2):25-29,5.基金项目
湖北省自然科学基金资助项目(2014CFB914) （2014CFB914）
中央高校基本科研业务费专项(CZW15112) （CZW15112）
