中南民族大学学报（自然科学版）2017，Vol.36Issue(2)：30-34,5.
角蛋白高效降解菌Fea-10的筛选与鉴定
Isolation and Identification of a High Efficient Feather-Degrading Strain Fea-10
摘要
Abstract
A high efficient keratin-degrading strain (Fea-10) was isolated from decomposed feathers by combining the method of clearance zone diameter and complete feather degradation.16S rDNA sequence similarity analysis showed that strain Fea-10 belonged to the genus Streptomyces with the highest sequence similarity to Streptomyces albidoflavus.Combined with morphological, physiological characteristics and phylogenetic tree analysis, the strain was then designated as Streptomyces albidoflavus Fea-10.Strain Fea-10 had one linear chromosome of 7.25 Mb with a G + C content of 73.36%.Analysis of the genome sequence of Fea-10 revealed that it contained abundant secondary biosynthesis gene cluster.The keratinase activity reached to the maximum value after fermenting for 36 hours with an optimum culture temperature of 35 ℃.关键词
角蛋白降解/微白黄链霉菌Fea-10/角蛋白酶/菌株筛选Key words
Keratin-degrading/Streptomyces albidoflavus Fea-10/keratinase/strain screening分类
化学化工引用本文复制引用
颜华,柯欣,吴佳,杨文翰,刘亚勇,贾良辉,马怡茗..角蛋白高效降解菌Fea-10的筛选与鉴定[J].中南民族大学学报（自然科学版）,2017,36(2):30-34,5.基金项目
陕西省农业科技创新与攻关项目(2015NY066),中央高校基本科研业务费项目(Z109021426、Z109021432) （2015NY066）
