中南民族大学学报（自然科学版）2017，Vol.36Issue(2)：45-48,4.
HPLC法同时测定不同产地龙血竭中3种黄酮类成分
Simultaneous Determination of Three kinds of Flavonoids in Resina Draconis from Different regions by HPLC
摘要
Abstract
To establish a method for the determination of loureirin A, loureirin B and cochinchinenin B in Resina Draconis from Guangxi and Yunnan Province in China, HPLC testing was performed on a Thermo BDS Hypersil C18 column (4.6 mm×250 mm) with mobile phases of acetonitrile-1% acetic acid solution in a gradient mode, with a flow rate of 1.0 mL·min-1, column temperature of 40℃, and detection wavelength of 278 nm.The three components were baseline separated in 45 mins and showed good linear relationship.The average content of loureirin A, loureirin B and cochinchinenin B in the nine batches of Resina Draconis were 0.47%, 0.96%, 0.44%, and the average recover rates were 99.6% (RSD=1.20%), 103.2% (RSD=0.65%), 100.5% (RSD=1.30%), respectively.This method was convenient, accurate and reliable, which could provide reference for the quality control of Resina Draconis.关键词
龙血竭/龙血素A/龙血素B/剑叶龙血素B/HPLC法Key words
Resina Draconis/loureirin A/loureirin B/cochinchinenin B/HPLC分类
化学化工引用本文复制引用
李竣,朱功俊,陈素,刘向明,李芸芳,杨光忠..HPLC法同时测定不同产地龙血竭中3种黄酮类成分[J].中南民族大学学报（自然科学版）,2017,36(2):45-48,4.基金项目
国家自然科学基金资助项目(81403186),湖北省高等学校优秀中青年科技创新团队计划资助项目(T201220),中央高校基本科研业务费专项(3212015CZW15029,3212015YCZW15095),中南民族大学实验室开放与技改资助项目(KF2016005,JG2016004) （81403186）
