作物研究2017，Vol.31Issue(3)：246-255,10.DOI:10.16848/j.cnki.issn.1001-5280.2017.03.08
甘蓝型油菜PPR家族生物信息学分析与新疆野生油菜候选育性基因克隆
Bioinformatics Analysis of PPR Family in Brassica napus L.and Cloning of Candidate Restorer Gene of Xinjiang Wild Rapeseed
摘要
Abstract
In this study,1079 protein sequences of PPR family were obtained from the genome of Brassica napus by Hidden Markov model.The motifs defined in the PPR family of Arabidopsis thaliana was used to classify them.The clustering,chromosome distribution,subcellular localization prediction,function Comments and so on were analyzed.Combining the molecular markers of the CMS line and the restorer line,the PPR gene GSBRNA2T00094406001 (named BnPPR_C09) located on C09 chromosome was screened for the potential fertility regulation gene of Xinjiang wild rapeseed.A cDNA sequence with length of 2514 bp was cloned from the CMS line 1193A of Xinjiang wild rapeseed and the restorer line 1193R by molecular cloning.Sequence analysis showed that the BnPPR_C09 gene (BnPPR_C09b) derived from 1193A had a single base deletion at + 1725 bp compared with the BnPPR_C09a derived from l193R,resulting in frameshift mutations.The bioinformatics analysis about predicted protein showed that the frameshift mutations caused termination of the translation process at + 1800 in the N-terminal of the BnPPR_C09b protein,and the subsequent deletion of a large number of functional components,the mutation may determine the fertility of Xinjiang wild rapeseed.关键词
新疆野生油菜/生物信息学/育性/基因克隆Key words
Xinjiang wild rapeseed/bioinformatics/fertility/gene clone分类
生物科学引用本文复制引用
谭晖,官春云..甘蓝型油菜PPR家族生物信息学分析与新疆野生油菜候选育性基因克隆[J].作物研究,2017,31(3):246-255,10.基金项目
国家重点研发计划(2016YFD010301) （2016YFD010301）
湖南省省长专项(湘财农指2016114号) （湘财农指2016114号）
湖南省科技计划(2013FJ4025). （2013FJ4025）
