作物研究2017，Vol.31Issue(3)：256-259,4.DOI:10.16848/j.cnki.issn.1001-5280.2017.03.09
拟南芥HGO基因启动子与GUS融合表达载体的构建及转化鉴定
Cloning and Transformation of the HGO Promoter from Arabidopsis thaliana
摘要
Abstract
The HGO gene encodes the 1,2-homogentisate dioxygenase,which catalyzes the last third step in the Tyr degradation pathway.In this study,a fusion expression vector of Arabidopsis thaliana HGO gene promoter and GUS gene was constructed and transformed into Arabidopsis thaliana by Agrobacterium-mediated transformation.Results from histochemical GUS assay suggested that fusion expression vector of HGO gene promoter and GUS gene was successfully constructed and it drives the expression of GUS gene successfully.Our results create valuable materials for studying the expression characteristics of HGO gene in Arabidopsis thaliana.关键词
拟南芥/HGO/启动子/同源重组/基因克隆Key words
Arabidopsis thaliana/HGO/promoter/homologous recombination/gene cloning分类
生物科学引用本文复制引用
蔡薇,支添添,任春梅..拟南芥HGO基因启动子与GUS融合表达载体的构建及转化鉴定[J].作物研究,2017,31(3):256-259,4.基金项目
国家973计划前期研究专项(2014CB160308). （2014CB160308）
