植物营养与肥料学报2017，Vol.23Issue(3)：821-826,6.DOI:10.11674/zwyf.16407
外源硫酸铝调节八仙花花青苷组成和含量变化的分子生物学机制
Mechanism of exogenous Al2(SO4)3 on regulating the anthocyanin concentration in Hydrangea macrophylla petal
摘要
Abstract
[Objectives] Hydrangea macrophylla is one of the most important ornamental plants.The present paper aimed to study effects of Al2(SO4)3 on flower colour of Hydrangea macrophylla.[Methods] Hydrangea macrophylla cultivar ‘mama blue’ was selected as material,and a pot experiment was conducted by setting up two A12(SO4)3 concentration levels,2‰ and 4‰.A12(SO4)3 was added into the growth media when the flower bud was about 1 cm length,and citrate buffer (pH 6.0) was added in control plants.The composition and concentrations of anthocyanins were determined by HPLC and mass spectrometry,ion concentrations were measured by ICP-AES,and expression of A13+ transport related genes was clarified by qRT-PCR.[Results]The petal colour changed from pink to purple and blue-purple after 21 d by the 2‰ and 4‰ A12(SO4)3 application,respectively.Twelve kinds of anthocyanins,such as delphinidin 3-glucoside,were detected in the petal.The A12(SO4)3 treatments increased the anthocyanins contents,especially the delphinidinderivatives contents.The delphinidinderivatives contents in petals were increased from 5159.9 μg/g FW in control plants to 24681.2 μg/g FW and 30485.7 pg/g FW in 2‰ and 4‰A12(SO4)3 treated plants,respectively.The increase of delphinidin derivatives contents was due to the enhancement of delphinidin 3-glucoside and delphinidin 3-pentose-5-glucoside.The delphinidin 3-glucoside contents in petals were increased from 4679.2 μg/g FW in control plants to 23610.0 μg/g FW and 29129.7μg/g FW in 2‰ and 4‰ A12(SO4)3 treated plants,respectively,and the contents of delphinidin 3-pentose-5-glucoside in petals were increased from 142.3 μg/g FW in control plants to 805.6 μg/g FW and 1114.9 μg/g FW in 2‰ and 4‰ A12(SO4)3 treated plants,respectively.And the Al3+ contents in petals were increased from 2.24 μg/g FW in control plants to 5.12 μg/g FW and 11.83 μg/g FW in 2‰ and 4‰ A12(SO4)3 treated plants,respectively.Accordingly,the gene expression of vacuolar Al transporter (VALT) and plasma membrane Al transporter (PALT) were increased significantly (P ＜ 0.05) by the A12(SO4)3 application.Compared with control plants,the PAL T expression levels were increased by 88.5％ and 148.2％ in 2‰ and 4‰ A12(SO4)3 treated plants,respectively,and the VAL T expression levels were increased by 74.8％ and 135.7％.[Conclusions] Our results suggested that A12(SO4)3 application induced the gene expression of VALT and PALT,enhanced the accumulation of Al3+,increased the delphinidinderivatives contents,and thus changed the flower colour from pink to purple and blue-purple.关键词
八仙花/花色/硫酸铝/花青苷/基因表达Key words
Hydrangea macrophylla/flower colour/A12(SO4)3/anthocyanin/gene expression引用本文复制引用
龚仲幸,何勇,杨静,宋亚,叶真逍,朱祝军..外源硫酸铝调节八仙花花青苷组成和含量变化的分子生物学机制[J].植物营养与肥料学报,2017,23(3):821-826,6.基金项目
浙江省自然科学基金重点项目(LZ14C150001)资助. （LZ14C150001）
